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AB164628

Recombinant Human GLYR1 protein (GST tag N-Terminus)

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Recombinant Human GLYR1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 484 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

HIBDL, NDF, NP60, NPAC, GLYR1, Cytokine-like nuclear factor N-PAC, 3-hydroxyisobutyrate dehydrogenase-like protein, Glyoxylate reductase 1 homolog, Nuclear protein NP60, Nuclear protein of 60 kDa, Nucleosome-destabilizing factor, Putative oxidoreductase GLYR1, hNDF

1 Images
SDS-PAGE - Recombinant Human GLYR1 protein (GST tag N-Terminus) (AB164628)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GLYR1 protein (GST tag N-Terminus) (AB164628)

ab164628 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q49A26

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Cell Lines & Lysates

AB266610

Human GLYR1 knockout HEK-293T cell line

Advanced Validation

Western blot - Human GLYR1 knockout HEK-293T cell line (AB266610)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB159886

Recombinant Human ZP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

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  • 0
Primary Antibodies

AB167155

Anti-GLYR1 antibody [EPR10077(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10077(B)] (AB167155)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MIKINKGKRFQQAVDAVEEFLRRAKGKDQTSSHNSSDDKNRRNSSEERSRPNSGDEKRKLSLSEGKVKKNMGEGKKRVSSGSSERGSKSPLKRAQEQSPRKRGRPPKDEKDLTIPESSTVKGMMAGPMAAFKWQPTASEPVKDADPHFHHFLLSQTEKPAVCYQAITKKLKICEEETGSTSIQAADSTAVNGSITPTDKKIGFLGLGLMGSGIVSNLLKMGHTVTVWNRTAEKCDLFIQEGARLGRTPAEVVSTCDITFACVSDPKAAKDLVLGPSGVLQGIRPGKCYVDMSTVDADTVTELAQVIVSRGGRFLEAPVSGNQQLSNDGMLVILAAGDRGLYEDCSSCFQAMGKTSFFLGEVGNAAKMMLIVNMVQGSFMATIAEGLTLAQVTGQSQQTLLDILNQGQLASIFLDQKCQNILQGNFKPDFYLKYIQKDLRLAIALGDAVNHPTPMAAAANEVYKRAKALDQSDNDMSAVYRAYIH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":484,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q49A26","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLYR1 also known as glyoxylate reductase 1 homolog or NP60 is a 62 kDa protein. It is an NAD(P)H-dependent oxidoreductase and binds specifically to histones modifying their methylation status. GLYR1 shows expression in various tissues with higher levels noted in the kidney and liver. It plays a role in chromatin remodeling indicating its involvement in gene expression regulation.
Biological function summary

GLYR1 influences chromatin structure by regulating histone H3 lysine 36 trimethylation (H3K36me3). It interacts with lysine-specific demethylase 1 (LSD1) and partners within chromatin-modifying complexes. Through these interactions GLYR1 can impact transcriptional repression and activation. Its activity shows specific importance in controlling gene expression related to cell growth and differentiation.

Pathways

GLYR1 interacts with components of the MAPK signaling pathway and the histone modification pathway. It works alongside proteins like LSD1 and histone deacetylases (HDACs) to modulate transcriptional outcomes. These interactions provide evidence of its role in transmitting extracellular signals to the nucleus ultimately influencing gene expression patterns.

Research links GLYR1 to cancer progression and inflammatory responses. Its overexpression or dysregulation has associations with tumors where alteration of histone marks leads to aberrant gene expression. GLYR1 also connects with proteins like HDACs in these diseases contributing to the modulation of cell cycle and apoptosis which are critical for cancer development.
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Specifications

Form

Liquid

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General info

Function

Cytokine-like nuclear factor with chromatin gene reader activity involved in chromatin modification and regulation of gene expression (PubMed : 23260659, PubMed : 30970244). Acts as a nucleosome-destabilizing factor that is recruited to genes during transcriptional activation (PubMed : 29759984, PubMed : 30970244). Recognizes and binds histone H3 without a preference for specific epigenetic markers and also binds DNA (PubMed : 20850016, PubMed : 30970244). Interacts with KDM1B and promotes its histone demethylase activity by facilitating the capture of H3 tails, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes (PubMed : 23260659, PubMed : 29759984, PubMed : 30970244). Stimulates the acetylation of 'Lys-56' of nucleosomal histone H3 (H3K56ac) by EP300 (PubMed : 29759984). With GATA4, co-binds a defined set of heart development genes and coregulates their expression during cardiomyocyte differentiation (PubMed : 35182466). Regulates p38 MAP kinase activity by mediating stress activation of MAPK14/p38alpha and specifically regulating MAPK14 signaling (PubMed : 16352664). Indirectly promotes phosphorylation of MAPK14 and activation of ATF2 (PubMed : 16352664). The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6 (PubMed : 16352664).

Sequence similarities

Belongs to the HIBADH-related family. NP60 subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Cytokine-like nuclear factor with chromatin gene reader activity involved in chromatin modification and regulation of gene expression (PubMed : 23260659, PubMed : 30970244). Acts as a nucleosome-destabilizing factor that is recruited to genes during transcriptional activation (PubMed : 29759984, PubMed : 30970244). Recognizes and binds histone H3 without a preference for specific epigenetic markers and also binds DNA (PubMed : 20850016, PubMed : 30970244). Interacts with KDM1B and promotes its histone demethylase activity by facilitating the capture of H3 tails, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes (PubMed : 23260659, PubMed : 29759984, PubMed : 30970244). Stimulates the acetylation of 'Lys-56' of nucleosomal histone H3 (H3K56ac) by EP300 (PubMed : 29759984). With GATA4, co-binds a defined set of heart development genes and coregulates their expression during cardiomyocyte differentiation (PubMed : 35182466). Regulates p38 MAP kinase activity by mediating stress activation of MAPK14/p38alpha and specifically regulating MAPK14 signaling (PubMed : 16352664). Indirectly promotes phosphorylation of MAPK14 and activation of ATF2 (PubMed : 16352664). The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6 (PubMed : 16352664).
See full target information GLYR1
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