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AB233610

Recombinant Human GM2A protein (6x His tag C-Terminus)

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Recombinant Human GM2A protein (6x His tag C-Terminus) is a Human Full Length protein, in the 26 to 193 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB, Mass Spec.

View Alternative Names

Ganglioside GM2 activator, Cerebroside sulfate activator protein, GM2-AP, Sphingolipid activator protein 3, SAP-3, GM2A

1 Images
SDS-PAGE - Recombinant Human GM2A protein (His tag) (AB233610)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GM2A protein (His tag) (AB233610)

SDS-PAGE analysis of ab233610 stained with Coomassie Blue. Right lane shows immunobloting with anti-6xHis monoclonal.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag C-Terminus

Applications

Mass Spec, WB, SDS-PAGE

applications

Biologically active

No

Accession

P17900

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 3.4% Imidazole Constituents: 2.9% Sodium chloride, 0.32% Tris HCl, 0.01% 2-Mercaptoethanol

storage-buffer

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Complementary Products

Proteins & Peptides

AB235716

Recombinant Human GBA protein

SDS-PAGE - Recombinant Human GBA protein (AB235716)

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Primary Antibodies

AB313587

Anti-GM2A antibody [EPR28371-75]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM2A antibody [EPR28371-75] (AB313587)

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Primary Antibodies

AB125065

Anti-GBA antibody [EPR5142]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Frozen sections) - Anti-GBA antibody [EPR5142] (AB125065)

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Primary Antibodies

AB23942

Anti-Ganglioside asialo GM2 antibody

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  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as GM2-AP
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Sequence info

[{"linker":null,"sequence":"KKPSQLSSFSWDNCDEGKDPAVIRSLTLEPDPIIVPGNVTLSVMGSTSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDLELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI","proteinLength":"Full Length","predictedMolecularWeight":"19.32 kDa","actualMolecularWeight":null,"aminoAcidEnd":193,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P17900","tags":[{"tag":"6x His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The human GM2A also known as GM2 activator protein functions mechanically as a lysosomal lipid transfer protein. It possesses a mass of approximately 23 kDa. It is expressed mainly in the tissues that have a high lysosomal activity like the brain. It binds specific glycolipids such as GM2 ganglioside facilitating their interaction with enzymatic components in the lysosome.
Biological function summary

The GM2A protein belongs to a family of proteins that assist in the degradation of complex lipids. It is an essential component of the ganglioside degradation process where it acts as a cofactor rather than being part of any complex. GM2A helps transfer lipids to enzymes such as β-hexosaminidase A ensuring proper breakdown and recycling of gangliosides particularly in neuronal tissues.

Pathways

GM2A plays an important role in the lysosomal degradation pathway specifically involving lipid metabolism. The degradation of gangliosides utilizes GM2A to facilitate interaction between GM2 ganglioside and proteins such as β-hexosaminidase A a necessary step in breaking down these complex lipids. Another significant pathway relates to its role in maintaining the balance of sphingolipid metabolism highlighting its collaboration with enzymes involved in this process.

Impaired activity or mutation in GM2A can lead to lipid storage diseases notably Tay-Sachs disease. Errors in GM2A disrupt the breakdown of gangliosides resulting in harmful accumulation within lysosomes. This dysfunction also involves the β-hexosaminidase A enzyme reinforcing the link between these proteins in the pathological buildup of gangliosides associated with Tay-Sachs disease.
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Specifications

Form

Liquid

Additional notes

Purified by 6xHis tag / Immobilized-metal affinity chromatography (IMAC).

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General info

Function

The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity (By similarity). Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3 (By similarity). Has cholesterol transfer activity (PubMed : 17552909).

Post-translational modifications

The serines in positions 32 and 33 are absent in 80% of the sequenced protein.

Subcellular localisation

Lysosome

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Product protocols

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Target data

The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity (By similarity). Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3 (By similarity). Has cholesterol transfer activity (PubMed : 17552909).
See full target information Ganglioside GM2 activator
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Product promise

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