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Recombinant Human GMDS protein is a Human Full Length protein, in the 1 to 372 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.

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Images

SDS-PAGE - Recombinant Human GMDS protein (AB113860), expandable thumbnail

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M A H A P A R C P S A R G S G D G E M G K P R N V A L I T G I T G Q D G S Y L A E F L L E K G Y E V H G I V R R S S S F N T G R I E H L Y K N P Q A H I E G N M K L H Y G D L T D S T C L V K I I N E V K P T E I Y N L G A Q S H V K I S F D L A E Y T A D V D G V G T L R L L D A V K T C G L I N S V K F Y Q A S T S E L Y G K V Q E I P Q K E T T P F Y P R S P Y G A A K L Y A Y W I V V N F R E A Y N L F A V N G I L F N H E S P R R G A N F V T R K I S R S V A K I Y L G Q L E C F S L G N L D A K R D W G H A K D Y V E A M W L M L Q N D E P E D F V I A T G E V H S V R E F V E K S F L H I G K T I V W E G K N E N E V G R C K E T G K V H V T V D L K Y Y R P T E V D F L Q G D C T K A K Q K L N W K P R V A F D E L V R E M V H A D V E L M R T N P N A

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.

Alternative names

Recommended products

Recombinant Human GMDS protein is a Human Full Length protein, in the 1 to 372 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Accession
O60547-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M A H A P A R C P S A R G S G D G E M G K P R N V A L I T G I T G Q D G S Y L A E F L L E K G Y E V H G I V R R S S S F N T G R I E H L Y K N P Q A H I E G N M K L H Y G D L T D S T C L V K I I N E V K P T E I Y N L G A Q S H V K I S F D L A E Y T A D V D G V G T L R L L D A V K T C G L I N S V K F Y Q A S T S E L Y G K V Q E I P Q K E T T P F Y P R S P Y G A A K L Y A Y W I V V N F R E A Y N L F A V N G I L F N H E S P R R G A N F V T R K I S R S V A K I Y L G Q L E C F S L G N L D A K R D W G H A K D Y V E A M W L M L Q N D E P E D F V I A T G E V H S V R E F V E K S F L H I G K T I V W E G K N E N E V G R C K E T G K V H V T V D L K Y Y R P T E V D F L Q G D C T K A K Q K L N W K P R V A F D E L V R E M V H A D V E L M R T N P N A

Accession

O60547

Protein length

Full Length

Predicted molecular weight

44.1 kDa

Amino acids

1 to 372

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

ab113860 was purified using conventional chromatography.

General info

Function

Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.

Sequence similarities

Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The GMDS protein also known as GDP-mannose 46-dehydratase plays an important role in catalyzing the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose one step in the biosynthesis of GDP-fucose. It has an approximate mass of 39 kDa and predominantly expresses in the liver kidneys and certain immune cells. The GMDS enzyme operates in the cytosol contributing to cellular metabolism and energy balance providing substrates necessary for further enzymatic conversions.

Biological function summary

GMDS contributes significantly to the fucosylation process a modification critical for the functioning of various glycoproteins and glycolipids. It participates in the synthesis of fucose which serves as a substrate for fucosyltransferases in glycan decorations. The enzymatic activity of GMDS occurs as part of a greater network within the cytosol although it does not directly involve multi-protein complexes. Its product GDP-fucose plays a role in cell-cell interaction and communication emphasizing GMDS’s importance in cell biology and molecular signaling.

Pathways

GMDS contributes to the fucose biosynthesis pathway and indirectly affects the Leloir pathway of galactose metabolism due to interconnected metabolic pools. Through its product GDP-fucose GMDS links to proteins such as fucosyltransferases which incorporate fucose into glycan structures. The availability of fucose also affects signaling pathways involving Notch receptor activation influencing cellular differentiation and growth making GMDS an essential player in glycan-related signal transduction.

Associated diseases and disorders

GMDS alterations have connections with immunodeficiencies and various cancer types. Deficiencies in GMDS can lead to Congenital Disorder of Glycosylation Type Iic where improper glycosylation affects immune cell functions and fucosylation imbalance links to increased tumor progression and metastasis through altered cell surface glycan structures. In the context of these disorders GMDS influences proteins such as alpha-13-fucosyltransferases with fucose content affecting functional glycan patterns on cells demonstrating its importance in pathophysiology related to glycosylation disorders.

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1 product image

  • SDS-PAGE - Recombinant Human GMDS protein (ab113860), expandable thumbnail

    SDS-PAGE - Recombinant Human GMDS protein (ab113860)

    15% SDS-PAGE analysis of ab113860 (3μg)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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