Recombinant Human GMDS protein is a Human Full Length protein, in the 1 to 372 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
>95% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, MS
No
M G S S H H H H H H S S G L V P R G S H M A H A P A R C P S A R G S G D G E M G K P R N V A L I T G I T G Q D G S Y L A E F L L E K G Y E V H G I V R R S S S F N T G R I E H L Y K N P Q A H I E G N M K L H Y G D L T D S T C L V K I I N E V K P T E I Y N L G A Q S H V K I S F D L A E Y T A D V D G V G T L R L L D A V K T C G L I N S V K F Y Q A S T S E L Y G K V Q E I P Q K E T T P F Y P R S P Y G A A K L Y A Y W I V V N F R E A Y N L F A V N G I L F N H E S P R R G A N F V T R K I S R S V A K I Y L G Q L E C F S L G N L D A K R D W G H A K D Y V E A M W L M L Q N D E P E D F V I A T G E V H S V R E F V E K S F L H I G K T I V W E G K N E N E V G R C K E T G K V H V T V D L K Y Y R P T E V D F L Q G D C T K A K Q K L N W K P R V A F D E L V R E M V H A D V E L M R T N P N A
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.
GDP-D-mannose dehydratase, GMD, GMDS
Recombinant Human GMDS protein is a Human Full Length protein, in the 1 to 372 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
>95% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, MS
No
No
Human
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
M G S S H H H H H H S S G L V P R G S H M A H A P A R C P S A R G S G D G E M G K P R N V A L I T G I T G Q D G S Y L A E F L L E K G Y E V H G I V R R S S S F N T G R I E H L Y K N P Q A H I E G N M K L H Y G D L T D S T C L V K I I N E V K P T E I Y N L G A Q S H V K I S F D L A E Y T A D V D G V G T L R L L D A V K T C G L I N S V K F Y Q A S T S E L Y G K V Q E I P Q K E T T P F Y P R S P Y G A A K L Y A Y W I V V N F R E A Y N L F A V N G I L F N H E S P R R G A N F V T R K I S R S V A K I Y L G Q L E C F S L G N L D A K R D W G H A K D Y V E A M W L M L Q N D E P E D F V I A T G E V H S V R E F V E K S F L H I G K T I V W E G K N E N E V G R C K E T G K V H V T V D L K Y Y R P T E V D F L Q G D C T K A K Q K L N W K P R V A F D E L V R E M V H A D V E L M R T N P N A
Full Length
44.1 kDa
1 to 372
Recombinant
His tag N-Terminus
Liquid
ab113860 was purified using conventional chromatography.
Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.
Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This supplementary information is collated from multiple sources and compiled automatically.
The GMDS protein also known as GDP-mannose 46-dehydratase plays an important role in catalyzing the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose one step in the biosynthesis of GDP-fucose. It has an approximate mass of 39 kDa and predominantly expresses in the liver kidneys and certain immune cells. The GMDS enzyme operates in the cytosol contributing to cellular metabolism and energy balance providing substrates necessary for further enzymatic conversions.
GMDS contributes significantly to the fucosylation process a modification critical for the functioning of various glycoproteins and glycolipids. It participates in the synthesis of fucose which serves as a substrate for fucosyltransferases in glycan decorations. The enzymatic activity of GMDS occurs as part of a greater network within the cytosol although it does not directly involve multi-protein complexes. Its product GDP-fucose plays a role in cell-cell interaction and communication emphasizing GMDS’s importance in cell biology and molecular signaling.
GMDS contributes to the fucose biosynthesis pathway and indirectly affects the Leloir pathway of galactose metabolism due to interconnected metabolic pools. Through its product GDP-fucose GMDS links to proteins such as fucosyltransferases which incorporate fucose into glycan structures. The availability of fucose also affects signaling pathways involving Notch receptor activation influencing cellular differentiation and growth making GMDS an essential player in glycan-related signal transduction.
GMDS alterations have connections with immunodeficiencies and various cancer types. Deficiencies in GMDS can lead to Congenital Disorder of Glycosylation Type Iic where improper glycosylation affects immune cell functions and fucosylation imbalance links to increased tumor progression and metastasis through altered cell surface glycan structures. In the context of these disorders GMDS influences proteins such as alpha-13-fucosyltransferases with fucose content affecting functional glycan patterns on cells demonstrating its importance in pathophysiology related to glycosylation disorders.
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15% SDS-PAGE analysis of ab113860 (3μg)
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