Recombinant Human GMPR1 protein is a Human Full Length protein, in the 1 to 345 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M P R I D A D L K L D F K D V L L R P K R S S L K S R A E V D L E R T F T F R N S K Q T Y S G I P I I V A N M D T V G T F E M A A V M S Q H S M F T A I H K H Y S L D D W K L F A T N H P E C L Q N V A V S S G S G Q N D L E K M T S I L E A V P Q V K F I C L D V A N G Y S E H F V E F V K L V R A K F P E H T I M A G N V V T G E M V E E L I L S G A D I I K V G V G P G S V C T T R T K T G V G Y P Q L S A V I E C A D S A H G L K G H I I S D G G C T C P G D V A K A F G A G A D F V M L G G M F S G H T E C A G E V I E R N G R K L K L F Y G M S S D T A M N K H A G G V A E Y R A S E G K T V E V P Y K G D V E N T I L D I L G G L R S T C T Y V G A A K L K E L S R R A T F I R V T Q Q H N T V F S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides.
Alternative names
GMPR1, GMPR, GMP reductase 1, GMPR 1, Guanosine 5'-monophosphate oxidoreductase 1, Guanosine monophosphate reductase 1
Recommended products
Recombinant Human GMPR1 protein is a Human Full Length protein, in the 1 to 345 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P36959-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M P R I D A D L K L D F K D V L L R P K R S S L K S R A E V D L E R T F T F R N S K Q T Y S G I P I I V A N M D T V G T F E M A A V M S Q H S M F T A I H K H Y S L D D W K L F A T N H P E C L Q N V A V S S G S G Q N D L E K M T S I L E A V P Q V K F I C L D V A N G Y S E H F V E F V K L V R A K F P E H T I M A G N V V T G E M V E E L I L S G A D I I K V G V G P G S V C T T R T K T G V G Y P Q L S A V I E C A D S A H G L K G H I I S D G G C T C P G D V A K A F G A G A D F V M L G G M F S G H T E C A G E V I E R N G R K L K L F Y G M S S D T A M N K H A G G V A E Y R A S E G K T V E V P Y K G D V E N T I L D I L G G L R S T C T Y V G A A K L K E L S R R A T F I R V T Q Q H N T V F S
- Accession
- P36959
- Protein length
- Full Length
- Predicted molecular weight
- 39.5 kDa
- Amino acids
- 1 to 345
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab123163 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides.
- Sequence similarities
Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The GMPR1 protein also known as guanosine monophosphate reductase 1 acts mechanistically as an enzyme that catalyzes the conversion of guanosine monophosphate (GMP) to inosine monophosphate (IMP). This reaction is part of the purine nucleotide cycle important for maintaining the balance of nucleotide pools in cells. GMPR1 has a molecular mass of approximately 37 kDa. Expression of GMPR1 is detected in various tissues with significant levels present in liver and kidney tissues.
- Biological function summary
GMPR1 plays a critical role in purine metabolism by regulating the intracellular concentrations of guanine nucleotides. It does not work as part of a larger complex but functions independently to achieve its effects on nucleotide turnover. This protein ensures efficient recycling of guanine nucleotides which is important for cellular processes such as DNA and RNA synthesis signalling and energy metabolism.
- Pathways
GMPR1 is involved mainly in the purine salvage pathway and the nucleotide degradation pathway. Through these pathways GMPR1 links with other proteins like hypoxanthine-guanine phosphoribosyltransferase (HGPRT) which also participates in nucleotide salvage. The balance achieved in these pathways is essential for cellular proliferation and energy conservation highlighting GMPR1's role in overall cellular homeostasis.
- Associated diseases and disorders
GMPR1 has connections to conditions like gout and certain types of cancer where there is an imbalance in purine metabolism. The misregulation of GMPR1 potentially affecting the pathways it participates in may lead to excess uric acid production seen in gout or altered nucleotide metabolism in tumorigenesis. Additionally proteins such as HGPRT which interacts within the same purine pathways also exhibit associations with these disorders further illustrating the significant impact of purine metabolism in human health.
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1 product image
SDS-PAGE - Recombinant Human GMPR1 protein (ab123163)
15% SDS-PAGE analysis of ab123163 (3 µg).
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