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AB268596

Recombinant Human GNA11 protein (Tagged)

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Recombinant Human GNA11 protein (Tagged) is a Human Full Length protein, in the 1 to 359 aa range, expressed in Escherichia coli, with >70%, suitable for SDS-PAGE.

View Alternative Names

GA11, GNA11, Guanine nucleotide-binding protein subunit alpha-11, G alpha-11, G-protein subunit alpha-11, Guanine nucleotide-binding protein G(y) subunit alpha

1 Images
SDS-PAGE - Recombinant Human GNA11 protein (Tagged) (AB268596)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GNA11 protein (Tagged) (AB268596)

SDS-PAGE analysis of ab268596.

MW ~66 kDa.

Key facts

Purity

>70% SDS-PAGE

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P29992

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MTLESMMACCLSDEVKESKRINAEIEKQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGAGYSEEDKRGFTKLVYQNIFTAMQAMIRAMETLKILYKYEQNKANALLIREVDVEKVTTFEHQYVSAIKTLWEDPGIQECYDRRREYQLSDSAKYYLTDVDRIATLGYLPTQQDVLRVRVPTTGIIEYPFDLENIIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEDKILYSHLVDYFPEFDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":359,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P29992","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNA11 also known as G-protein subunit alpha-11 is a protein that functions mechanically within cells as part of the G protein family. This protein plays a role in transmitting signals from the surface of the cell to its interior. GNA11 has a molecular mass of approximately 42 kDa and it is mainly expressed in various tissues including the heart and central nervous system. It operates by interacting with receptors on the cell surface instigating conformational changes that initiate intracellular signaling cascades.
Biological function summary

G-protein subunit alpha-11 interacts with several signaling molecules and works predominantly as part of a heterotrimeric G protein complex. This protein complex plays an important role in modulating a variety of biological processes such as cardiovascular function and neuronal signaling. GNA11 contributes to cellular responses by activating downstream effectors which can influence cellular growth differentiation and response to external stimuli.

Pathways

G-protein subunit alpha-11 is an essential component of the phosphoinositide signaling pathway. It assists in the conversion of extracellular signals into intracellular action by facilitating the release of secondary messengers like inositol trisphosphate. In this pathway it works closely with other proteins like GNAQ and GNA15 which share similar mechanistic roles. Through these interactions GNA11 regulates calcium mobilization and protein kinase C activity impacting various cellular functions.

GNA11 mutations have links to certain conditions like uveal melanoma and hypocalcemia. These mutations alter the normal signaling pathways and can lead to unregulated cell growth as seen in cancer. Uveal melanoma relates to GNA11 through mutations that activate signaling pathways uncontrollably. Similarly in hypocalcemia the GNA11 protein associates with the calcium-sensing receptor which causes imbalances in calcium homeostasis. These associations highlight the significance of GNA11 in pathology as well as signaling pathways.
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Specifications

Form

Liquid

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General info

Function

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 31073061). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 31073061). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 31073061). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 31073061). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 31073061). Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation : activates phospholipase C-beta : following GPCR activation, GNA11 activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed : 31073061). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed : 27852822). Together with GNAQ, required for heart development (By similarity).

Sequence similarities

Belongs to the G-alpha family. G(q) subfamily.

Post-translational modifications

(Microbial infection) Deamidated at Gln-209 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.

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Product protocols

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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 31073061). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 31073061). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 31073061). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 31073061). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 31073061). Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation : activates phospholipase C-beta : following GPCR activation, GNA11 activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed : 31073061). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed : 27852822). Together with GNAQ, required for heart development (By similarity).
See full target information GNA11
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