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AB268597

Recombinant Human GNA13 protein (Tagged)

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(1 Publication)

Recombinant Human GNA13 protein (Tagged) is a Human Full Length protein, in the 1 to 377 aa range, expressed in Escherichia coli, with >70%, suitable for SDS-PAGE.

View Alternative Names

Guanine nucleotide-binding protein subunit alpha-13, G alpha-13, G-protein subunit alpha-13, GNA13

1 Images
SDS-PAGE - Recombinant Human GNA13 protein (Tagged) (AB268597)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GNA13 protein (Tagged) (AB268597)

SDS-PAGE analysis of ab268597.

MW ~67 kDa.

Key facts

Purity

>70% SDS-PAGE

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q14344

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MADFLPSRSVLSVCFPGCLLTSGEAEQQRKSKEIDKCLSREKTYVKRLVKILLLGAGESGKSTFLKQMRIIHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQQHGDKMMSFDTRAPMAAQGMVETRVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYIPSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLVECFRNKRRDQQQKPLYHHFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":377,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q14344","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNA13 also known as Gα13 is a member of the G12 family of G proteins. It has a molecular mass of approximately 44 kDa and functions as a guanine nucleotide-binding protein. This protein is important in transmitting signals from G protein-coupled receptors (GPCRs) to intracellular signaling pathways. GNA13 is widely expressed in various tissues including endothelial cells smooth muscle cells and the brain. The signaling capability of GNA13 is based on its ability to bind GTP and GDP functioning through a cycle of activation and inactivation.
Biological function summary

The role of G protein subunit GNA13 involves participating in critical cellular functions such as cytoskeletal reorganization cell migration and proliferation. It is an integral part of the G12/G13 subunits` complex which plays a role in controlling cell dynamics and morphology. Through these activities GNA13 contributes to the regulation of processes like embryonic development and tissue repair. Additionally it influences cell behavior through the RhoA pathway affecting actin cytoskeleton and cell shape changes.

Pathways

GNA13 interacts with a variety of important signaling systems. One primary pathway where GNA13 is active is the Rho signaling pathway where it activates RhoA leading to changes in the actin cytoskeleton. This pathway is necessary for cellular processes like adhesion and motility. Additionally GNA13 participates in the mitogen-activated protein kinase (MAPK) pathway influencing cellular proliferation. Through these pathways GNA13 is related to proteins such as RhoGEFs which act as guanine nucleotide exchange factors and MAPKs essential in transmitting mitogenic signals.

GNA13 has implications in cancer development and cardiovascular diseases. Its role in Rho signaling and cell motility can contribute to tumor progression and metastasis with alterations in its function linked to various cancers. Additionally in cardiovascular health GNA13's influence on vascular smooth muscle contraction can relate to conditions such as hypertension. Projecting its influence through RhoA GNA13's malfunction connects it to pathological changes within these disease contexts.
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Specifications

Form

Liquid

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General info

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed : 15240885, PubMed : 16705036, PubMed : 16787920, PubMed : 27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed : 12515866, PubMed : 15240885). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway (PubMed : 16705036, PubMed : 16787920, PubMed : 27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed : 11976333).

Sequence similarities

Belongs to the G-alpha family. G(12) subfamily.

Post-translational modifications

Palmitoylation is critical for proper membrane localization and signaling.. Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed : 15240885, PubMed : 16705036, PubMed : 16787920, PubMed : 27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed : 12515866, PubMed : 15240885). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway (PubMed : 16705036, PubMed : 16787920, PubMed : 27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed : 11976333).
See full target information Guanine nucleotide-binding protein subunit alpha-13
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108136 PubMed39730062

2024

Receptor-independent regulation of Gα13 by alpha-1-antitrypsin C-terminal peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghak Park,Shigeyuki Matsumoto,Kosuke Ogata,Biao Ma,Ryo Kanada,Yuta Isaka,Norihito Arichi,Xiaowen Liang,Ritsuko Maki,Tohru Kozasa,Yasushi Okuno,Hiroaki Ohno,Yasushi Ishihama,Fumiko Toyoshima
View all publications
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