Recombinant Human GNAQ protein is a Human Full Length protein, in the 1 to 359 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Publications
- American journal of physiology. Regulatory, integrative and comparative physiology 318:R38-R482019Evidence that hindbrain astrocytes in the rat detect low glucose with a glucose transporter 2-phospholipase C-calcium release mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard C Rogers,Susan J Burke,J Jason Collier,Sue Ritter,Gerlinda E HermannPubMed 31596114
Key facts
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
Amino acid sequence
M T L E S I M A C C L S E E A K E A R R I N D E I E R Q L R R D K R D A R R E L K L L L L G T G E S G K S T F I K Q M R I I H G S G Y S D E D K R G F T K L V Y Q N I F T A M Q A M I R A M D T L K I P Y K Y E H N K A H A Q L V R E V D V E K V S A F E N P Y V D A I K S L W N D P G I Q E C Y D R R R E Y Q L S D S T K Y Y L N D L D R V A D P A Y L P T Q Q D V L R V R V P T T G I I E Y P F D L Q S V I F R M V D V G G Q R S E R R K W I H C F E N V T S I M F L V A L S E Y D Q V L V E S D N E N R M E E S K A L F R T I I T Y P W F Q N S S V I L F L N K K D L L E E K I M Y S H L V D Y F P E Y D G P Q R D A Q A A R E F I L K M F V D L N P D S D K I I Y S H F T C A T D T E N I R F V F A A V K D T I L Q L N L K E Y N L V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs).
Alternative names
Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein alpha-q, GAQ, GNAQ
Recommended products
Recombinant Human GNAQ protein is a Human Full Length protein, in the 1 to 359 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Alternative names
Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein alpha-q, GAQ, GNAQ
Key facts
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Accession
- P50148-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
M T L E S I M A C C L S E E A K E A R R I N D E I E R Q L R R D K R D A R R E L K L L L L G T G E S G K S T F I K Q M R I I H G S G Y S D E D K R G F T K L V Y Q N I F T A M Q A M I R A M D T L K I P Y K Y E H N K A H A Q L V R E V D V E K V S A F E N P Y V D A I K S L W N D P G I Q E C Y D R R R E Y Q L S D S T K Y Y L N D L D R V A D P A Y L P T Q Q D V L R V R V P T T G I I E Y P F D L Q S V I F R M V D V G G Q R S E R R K W I H C F E N V T S I M F L V A L S E Y D Q V L V E S D N E N R M E E S K A L F R T I I T Y P W F Q N S S V I L F L N K K D L L E E K I M Y S H L V D Y F P E Y D G P Q R D A Q A A R E F I L K M F V D L N P D S D K I I Y S H F T C A T D T E N I R F V F A A V K D T I L Q L N L K E Y N L V
- Accession
- Protein length
Full Length
- Predicted molecular weight
65.23 kDa
- Amino acids
1 to 359
- Nature
Recombinant
Specifications
- Form
Liquid
General info
- Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs).
- Sequence similarities
Belongs to the G-alpha family. G(q) subfamily.
- Post-translational modifications
Palmitoylated by ZDHHC3 and ZDHHC7 (PubMed:19001095). Palmitoylation occurs in the Golgi and participates in the localization of GNAQ to the plasma membrane (PubMed:19001095).
- Subcellular localisation
Nucleus, Nucleus membrane
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
U+00A0;
U+00A0;
Supplementary info
- Activity summary
GNAQ also known as Guanine nucleotide-binding protein G(q) subunit alpha plays a role in cell signaling as a modulator or transducer in various transmembrane signaling systems. With a molecular weight of approximately 42 kDa GNAQ belongs to the Gq family of G proteins. It is widely expressed in human tissues such as the brain heart and placenta. The protein's main mechanism involves binding to GTP and GDP leading it to switch between inactive GDP-bound and active GTP-bound states.
- Biological function summary
GNAQ influences multiple cell processes mediating the activation of phospholipase C-beta a critical enzyme in hydrolyzing phosphatidylinositol 45-bisphosphate (PIP2) to inositol trisphosphate and diacylglycerol. This action leads to the release of calcium ions from intracellular stores. GNAQ acts as part of a heterotrimeric G protein complex which requires coordination with beta and gamma subunits to achieve full functional capability. Its activity is critical for processes like cell proliferation and differentiation.
- Pathways
GNAQ notably participates in the phosphoinositide signaling pathway and calcium signaling pathway. In the phosphoinositide signaling pathway GNAQ facilitates the link between G protein-coupled receptors (GPCRs) and downstream effectors such as phospholipase C-beta. Its activity couples with proteins like GNA11 another member of the Gq family which often shares overlapping functions and roles within these pathways. The calcium signaling pathway involves GNAQ-induced release of calcium ions contributing to various cellular events.
- Associated diseases and disorders
GNAQ mutations link to uveal melanoma and Sturge-Weber syndrome. Studies show that these mutations activate the MAPK pathway independently promoting uncontrolled cell growth seen in uveal melanoma. GNA11 closely related to GNAQ also frequently undergoes similar mutations in uveal melanoma. In Sturge-Weber syndrome mutation of GNAQ leads to abnormal capillary growth and neurological issues evidencing its pathogenic role in vascular malformations.
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1 product image
SDS-PAGE - Recombinant Human GNAQ protein (ab132889)
12.5% SDS-PAGE analysis of ab132889 stained with Coomassie Blue.
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