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AB268602

Recombinant human GNAS protein (Active)

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Recombinant human GNAS protein (Active) is a Human Full Length protein, in the 2 to 394 aa range, with >95% purity and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab268602 protein is 70 kDa.

- Save time and ensure accurate results - use our recombinant G protein alpha S(GNAS) protein as a control
- Optimal protein bioactivity, stability and reproducibility

View Alternative Names

GNAS1, GSP, GNAS, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Adenylate cyclase-stimulating G alpha protein

2 Images
Functional Studies - Recombinant human GNAS protein (Active) (AB268602)
  • FuncS

Supplier Data

Functional Studies - Recombinant human GNAS protein (Active) (AB268602)

The specific activity of GNAS was determined to be 38 nmol/min/mg in a GTPase-Glo assay using GNAS substrate.

SDS-PAGE - Recombinant human GNAS protein (Active) (AB268602)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human GNAS protein (Active) (AB268602)

SDS-PAGE analysis of ab268602.

MW ~70 kDa.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of GNAS was determined to be 38 nmol/min/mg in a GTPase-Glo assay using GNAS substrate.

Accession

P63092

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our bioactive recombinant human G protein alpha S (GNAS) protein ab268602 as a positive control in SDS-PAGE.

Check out our protein gel staining guide for SDS-PAGE here
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":394,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P63092","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GNAS protein also known as GNAS complex locus or guanine nucleotide-binding protein G(s) subunit alpha (Gαs) is a highly versatile target in cellular signaling. It has a molecular mass of approximately 45 kDa. Widely expressed across various tissues including those in the brain kidneys and endocrine glands GNAS protein plays an important role in transmitting signals from receptors on cell surfaces to effector systems inside cells.
Biological function summary

GNAS protein mediates the action of hormones and neurotransmitters by activating the cyclic AMP (cAMP) signaling pathway. It forms part of the G protein complex specifically the G protein-coupled receptors (GPCRs) where it acts as the alpha subunit interacting with beta and gamma subunits. This interaction facilitates the conversion of GDP to GTP leading to downstream activation of adenylate cyclase which increases intracellular cAMP levels and triggers various cellular responses.

Pathways

GNAS protein is pivotal in the cAMP signaling pathway and the MAPK/ERK pathway. Within these pathways it works closely with adenylate cyclase enzymes which further influence numerous physiological processes such as metabolism and cell growth. The protein’s activity also interlinks with other G proteins contributing to a wide range of cellular reactions and feedback mechanisms.

Mutations or dysregulation of the GNAS gene are associated with McCune-Albright syndrome and pseudohypoparathyroidism type 1a. McCune-Albright syndrome arises from somatic mutations in the GNAS gene that result in constitutively active Gαs protein leading to abnormal cellular proliferation. In pseudohypoparathyroidism the loss of GNAS protein function disrupts normal hormonal signaling causing resistance to parathyroid hormone (PTH) which impacts calcium and phosphate regulation in the body. The involvement of GNAS protein in these conditions highlights its key role in signal transduction and endocrine regulation.
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Specifications

Form

Liquid

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General info

Function

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs) (PubMed : 12391161, PubMed : 17110384, PubMed : 21488135, PubMed : 26206488, PubMed : 8702665, PubMed : 10200251). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP (PubMed : 17110384, PubMed : 26206488, PubMed : 26206488, PubMed : 8702665). Functions downstream of beta-adrenergic receptors (PubMed : 21488135). Stimulates the Ras signaling pathway via RAPGEF2 (PubMed : 12391161).

Sequence similarities

Belongs to the G-alpha family. G(s) subfamily.

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Product protocols

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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs) (PubMed : 12391161, PubMed : 17110384, PubMed : 21488135, PubMed : 26206488, PubMed : 8702665, PubMed : 10200251). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP (PubMed : 17110384, PubMed : 26206488, PubMed : 26206488, PubMed : 8702665). Functions downstream of beta-adrenergic receptors (PubMed : 21488135). Stimulates the Ras signaling pathway via RAPGEF2 (PubMed : 12391161).
See full target information GNAS
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