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AB171490

Recombinant Human GNB3 protein (denatured)

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Recombinant Human GNB3 protein (denatured) is a Human Full Length protein, in the 1 to 340 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE.

View Alternative Names

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, Transducin beta chain 3, GNB3

1 Images
SDS-PAGE - Recombinant Human GNB3 protein (denatured) (AB171490)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GNB3 protein (denatured) (AB171490)

15% SDS-PAGE analysis of 3µg ab171490.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P16520

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMGEMEQLRQEAEQLKKQIADARKACADVTLAELVSGLEVVGRVQMRTRRTLRGHLAKIYAMHWATDSKLLVSASQDGKLIVWDSYTTNKVHAIPLRSSWVMTCAYAPSGNFVACGGLDNMCSIYNLKSREGNVKVSRELSAHTGYLSCCRFLDDNNIVTSSGDTTCALWDIETGQQKTVFVGHTGDCMSLAVSPDFNLFISGACDASAKLWDVREGTCRQTFTGHESDINAICFFPNGEAICTGSDDASCRLFDLRADQELICFSHESIICGITSVAFSLSGRLLFAGYDDFNCNVWDSMKSERVGILSGHDNRVSCLGVTADGMAVATGSWDSFLKIWN","proteinLength":"Full Length","predictedMolecularWeight":"39.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":340,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P16520","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNB3 also known as Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 functions as an integral component of heterotrimeric G proteins. The protein has a mass of approximately 37 kDa. GNB3 is expressed in various tissues with significant expression in the brain and heart. It forms part of the G protein complex essential for transmitting signals from many hormones neurotransmitters and other signaling factors.
Biological function summary

GNB3 interacts with alpha and gamma subunits to mediate signal transduction from G protein-coupled receptors (GPCRs). It plays a role in sensory perception neurotransmission and regulation of ion channels and certain enzymes. GNB3 is part of the G protein complex which appears critical in maintaining cellular response and adaptation to external signals. The interaction with different G protein subunits enables diverse regulatory effects on cellular functions.

Pathways

GNB3 participates in the GPCR signaling pathway and the calcium signaling pathway. It interacts with G protein alpha subunits like GNAI1 and GNAS which are key players in these pathways. GPCR signaling affects numerous physiological processes involving aspects like cellular growth development and metabolic regulation. GNB3 contributes to converting the activated receptor signal into appropriate cellular responses.

Altered GNB3 function associates with essential hypertension and obesity. Studies show connections between specific GNB3 polymorphisms and these conditions sometimes indicating variations in response to antihypertensive treatments. GNB3 interacts with components of the signal transduction pathways like the protein PLCB1 which may further elucidate its role in disease mechanisms. Understanding these interactions could enhance therapeutic strategies targeting related diseases.
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Specifications

Form

Liquid

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General info

Function

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Sequence similarities

Belongs to the WD repeat G protein beta family.

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Product protocols

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Target data

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
See full target information Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
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Product promise

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