Recombinant Human GNGT1 protein is a Human Fragment protein, in the 1 to 71 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M P V I N I E D L T E K D K L K M E V D Q L K K E V T L E R M L V S K C C E E V R D Y V E E R S G E D P L V K G I P E D K N P F K E L K G G C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Alternative names
Guanine nucleotide-binding protein G(T) subunit gamma-T1, Transducin gamma chain, GNGT1
Recommended products
Recombinant Human GNGT1 protein is a Human Fragment protein, in the 1 to 71 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P63211-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.03% EDTA
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M P V I N I E D L T E K D K L K M E V D Q L K K E V T L E R M L V S K C C E E V R D Y V E E R S G E D P L V K G I P E D K N P F K E L K G G C
- Accession
- P63211
- Protein length
- Fragment
- Predicted molecular weight
- 10.6 kDa
- Amino acids
- 1 to 71
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab183256 is purified using conventional chromatography.
General info
- Function
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
- Sequence similarities
Belongs to the G protein gamma family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GNGT1 also known as guanine nucleotide-binding protein G(t) subunit gamma-1 is a critical component of the heterotrimeric G-protein complex. With a molecular mass of approximately 8 kDa GNGT1 is highly expressed in the retina and pineal gland. It plays an essential role in signal transduction processes by participating in the interaction with the alpha and beta subunits of the G-protein heterotrimer which is important for transmitting signals from activated receptors to intracellular effectors.
- Biological function summary
The gamma subunit's interaction with the beta subunit forms a stable dimer which is vital for the proper functioning of G-proteins. GNGT1 as part of this beta-gamma complex modulates the activity of specific receptors linked to neurotransmission and sensory reception. This complex's significance is realized in phototransduction within the retinal cells where it supports the transmission of visual signals by regulating cGMP levels and phosphodiesterase activity.
- Pathways
This protein plays a pivotal role in the phototransduction and Ras signaling pathways. In phototransduction GNGT1 contributes to the regulation of visual pigment signaling by interacting with transducin a protein related to the G(alpha) subunit of the pathway. It influences the biochemical cascade that enables the conversion of visual signals into nerve impulses. Moreover through Ras signaling GNGT1 influences pathways involved in cell growth extending its functional implications beyond sensory perception.
- Associated diseases and disorders
Mutations or dysregulation of GNGT1 have been implicated in retinitis pigmentosa and congenital stationary night blindness. These conditions reflect the protein's important role in retinal function and the visual cycle. Additionally the association between GNGT1 and transducin signals a pathway distortion in these diseases where improper signaling can lead to progressive retinal degeneration or impaired vision function. Understanding GNGT1 in these contexts is key for potential therapeutic targeting and intervention in retinal disorders.
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1 product image
SDS-PAGE - Recombinant Human GNGT1 protein (ab183256)
15% SDS PAGE analysis of ab183256 (3μg).
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