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AB101942

Recombinant Human GNPNAT1 protein

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Recombinant Human GNPNAT1 protein is a Human Full Length protein, in the 1 to 184 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

GNA1, GNPNAT1, Glucosamine 6-phosphate N-acetyltransferase, Phosphoglucosamine acetylase, Phosphoglucosamine transacetylase

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SDS-PAGE - Recombinant Human GNPNAT1 protein (AB101942)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human GNPNAT1 protein (AB101942)

15% SDS-PAGE analysis of 3μg ab101942.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q96EK6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMKPDETPMFDPSLLKEVDWSQNTATFSPAISPTHPGEGLVLRPLCTADLNRGFFKVLGQLTETGVVSPEQFMKSFEHMKKSGDYYVTVVEDVTLGQIVATATLIIEHKFIHSCAKRGRVEDVVVSDECRGKQLGKLLLSTLTLLSKKLNCYKITLECLPQNVGFYKKFGYTVSEENYMCRRFLK","proteinLength":"Full Length","predictedMolecularWeight":"23.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":184,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96EK6","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNPNAT1 also known as Glucosamine-phosphate N-acetyltransferase plays a mechanical role as an enzyme in the biosynthesis of UDP-N-acetylglucosamine. This enzyme facilitates the transfer of an acetyl group from acetyl-CoA to glucosamine-6-phosphate forming N-acetylglucosamine-6-phosphate. With a molecular mass of approximately 26 kDa GNPNAT1 expresses in various tissues with significant levels detected in the liver and kidney among other organs.
Biological function summary

GNPNAT1 participates in the hexosamine biosynthetic pathway functioning in cellular processes like protein glycosylation. This protein does not act alone; it interacts with other enzymes to complete its function. GNPNAT1 activity influences the supply of N-acetyl-D-glucosamine an essential substrate for glycoprotein and glycolipid synthesis integral for proper cell signaling and structural firmness.

Pathways

GNPNAT1 plays a critical role in metabolic pathways particularly influencing the hexosamine biosynthetic pathway. This pathway connects to several metabolic networks including the production of glycosaminoglycans. It orchestrates with enzymes like glutamine fructose-6-phosphate amidotransferase (GFAT) to regulate the flux of substrates into these pathways therefore impacting energy metabolism and nutrient sensing.

GNPNAT1 implicates in conditions such as cancer and metabolic disorders. Abnormalities in its expression or function can lead to increased risks of various cancer types possibly due to disrupted glycosylation pathways affecting cell growth and adhesion. In diabetes the relationship between GNPNAT1 and GFAT might influence the development of insulin resistance highlighting its impact on glucose metabolism regulation.
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Specifications

Form

Liquid

Additional notes

ab101942 is purified using conventional chromatography techniques.

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General info

Sequence similarities

Belongs to the acetyltransferase family. GNA1 subfamily.

Subcellular localisation

Endosome membrane

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Product protocols

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Target data

See full target information GNPNAT1
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