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AB151650

Recombinant Human GNS protein

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Recombinant Human GNS protein is a Human Full Length protein, in the 37 to 552 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

N-acetylglucosamine-6-sulfatase, Glucosamine-6-sulfatase, G6S, GNS

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, HPLC

applications

Biologically active

No

Accession

P15586

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab151650 was produced by a mammalian cell expression system in HEK293.
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Sequence info

[{"sequence":"VFGVAAGTRRPNVVLLLTDDQDEVLGGMTPLKKTKALIGEMGMTFSSAYVPSALCCPSRASILTGKYPHNHHVVNNTLEGNCSSKSWQKIQEPNTFPAILRSMCGYQTFFAGKYLNEYGAPDAGGLEHVPLGWSYWYALEKNSKYYNYTLSINGKARKHGENYSVDYLTDVLANVSLDFLDYKSNFEPFFMMIATPAPHSPWTAAPQYQKAFQNVFAPRNKNFNIHGTNKHWLIRQAKTPMTNSSIQFLDNAFRKRWQTLLSVDDLVEKLVKRLEFTGELNNTYIFYTSDNGYHTGQFSLPIDKRQLYEFDIKVPLLVRGPGIKPNQTSKMLVANIDLGPTILDIAGYDLNKTQMDGMSLLPILRGASNLTWRSDVLVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDAYNNTYACVRTMSALWNLQYCEFDDQEVFVEVYNLTADPDQITNIAKTIDPELLGKMNYRLMMLQSCSGPTCRTPGVFDPGYRFDPRLMFSNRGSVRTRRFSKHLLVDHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"58 kDa","actualMolecularWeight":null,"aminoAcidEnd":552,"aminoAcidStart":37,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P15586","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GNS protein also known as glucosamine (N-acetyl)-6-sulfatase is an enzyme with significant roles in the breakdown of glycosaminoglycans. Its molecular weight is around 63 kDa. GNS is frequently expressed in lysosomal compartments where it catalyzes the cleavage of sulfate groups from N-acetylglucosamine residues in heparan sulfate and other similar molecules. This process is critical for the degradation and recycling of complex carbohydrates in cells.
Biological function summary

Glucosamine (N-acetyl)-6-sulfatase activity influences cellular homeostasis by acting within the lysosomal hydrolase family. GNS participates in the breakdown and metabolism of glycosaminoglycans important for maintaining cellular integrity and signaling functions. It operates as a standalone enzyme rather than part of a larger complex. Through its enzymatic function GNS helps prevent the accumulation of partially degraded molecules inside lysosomes.

Pathways

Glucosamine (N-acetyl)-6-sulfatase activity plays a role in lysosomal storage pathways and in the catabolic processes of heparan sulfate. This enzyme works alongside other lysosomal proteins like iduronate sulfatase and alpha-L-iduronidase which are also involved in glycosaminoglycan degradation pathways. Proper function of these pathways is important for cellular waste management and nutrient recycling.

Altered GNS function relates to conditions such as mucopolysaccharidosis type IIID (Sanfilippo syndrome type D) and some forms of lysosomal storage diseases. Deficiencies in GNS disrupt normal lysosomal breakdown of glycosaminoglycans leading to accumulations that can cause cellular damage. This disorder often involves other metabolic proteins like heparan N-sulfatase and alpha-N-acetylglucosaminidase. Understanding the role of GNS offers insights into potential therapeutic approaches for related metabolic disorders.
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Specifications

Form

Liquid

Additional notes

ab151650 was determined to be >95% pure by SEC-HPLC and reducing SDS-PAGE.

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General info

Sequence similarities

Belongs to the sulfatase family.

Post-translational modifications

The form A (78 kDa) is processed by internal peptidase cleavage to a 32 kDa N-terminal species (form B) and a 48 kDa C-terminal species.. The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.

Subcellular localisation

Lysosome

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Product protocols

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Target data

See full target information N-acetylglucosamine-6-sulfatase
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Primary Antibodies

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Western blot - Anti-GNS antibody [EPR8329(2)] - BSA and Azide free (AB249077)

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Primary Antibodies

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Primary Antibodies

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