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AB275562

Recombinant Human GPBB protein (Tagged)

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Recombinant Human GPBB protein (Tagged) is a Human Fragment protein, in the 477 to 731 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

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PYGB

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SDS-PAGE - Recombinant Human GPBB protein (Tagged) (AB275562)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GPBB protein (Tagged) (AB275562)

SDS-PAGE analysis of ab275562.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His-T7 tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P11216

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS.

Storage buffer

pH: 8 Constituents: 56.75% Trehalose, 33.22% Sodium chloride, 9.53% Sodium bicarbonate, 0.11% Sodium-N-Lauroylsarcosinate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"PEKFQNKTNGITPRRWLLLCNPGLADTIVEKIGEEFLTDLSQLKKLLPLVSDEVFIRDVAKVKQENKLKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNARE","proteinLength":"Fragment","predictedMolecularWeight":"32.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":731,"aminoAcidStart":477,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P11216","tags":[{"tag":"His-T7","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein generally known as GPBB (glycogen phosphorylase brain form) plays a critical role in glucose metabolism. An alternate name for this protein is brain-type glycogen phosphorylase. The GPBB protein weighs approximately 97 kDa. It is mainly expressed in the brain but also present in skeletal and cardiac muscle tissues. This enzyme catalyzes the breakdown of glycogen into glucose-1-phosphate an essential step in energy production particularly during periods of increased cellular energy demand.
Biological function summary

GPBB contributes significantly to cellular energy homeostasis. It often partners with AMP-activated protein kinase (AMPK) among other proteins acting as a part of cellular complexes that regulate energy metabolism. GPBB is essential in maintaining the balance between glycogen synthesis and degradation ensuring that glucose levels rise during energy-demanding activities. This regulation supports proper brain function especially in neurons which heavily rely on glucose as an energy source.

Pathways

GPBB is fundamental in the glycogenolysis pathway which mobilizes glucose from glycogen reserves during metabolic needs. GPBB also relates to the insulin signaling pathway influencing how cells respond to insulin a protein vital for glucose uptake and metabolism. Through these pathways GPBB interacts with other proteins like glycogen synthase and phosphoglucomutase establishing a network that carefully coordinates metabolic responses to varying energy availability.

GPBB has significant implications in cardiac ischemia and neurodegenerative disorders such as Alzheimer's disease. During cardiac ischemia elevated levels of GPBB often indicate heart damage given its role in glycogen breakdown in cardiac muscle cells. In Alzheimer's disease abnormal GPBB activity might affect neuronal energy balance contributing to the disease's progression. Understanding these connections is important for developing therapeutic approaches targeting metabolic pathways involving GPBB.
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Specifications

Form

Lyophilized

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General info

Function

Glycogen phosphorylase that regulates glycogen mobilization (PubMed : 27402852). Phosphorylase is an important allosteric enzyme in carbohydrate metabolism (PubMed : 3346228). Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates (PubMed : 3346228). However, all known phosphorylases share catalytic and structural properties (PubMed : 3346228).

Sequence similarities

Belongs to the glycogen phosphorylase family.

Post-translational modifications

Phosphorylated (PubMed:27402852). Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A (By similarity).

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Product protocols

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Target data

Glycogen phosphorylase that regulates glycogen mobilization (PubMed : 27402852). Phosphorylase is an important allosteric enzyme in carbohydrate metabolism (PubMed : 3346228). Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates (PubMed : 3346228). However, all known phosphorylases share catalytic and structural properties (PubMed : 3346228).
See full target information Glycogen phosphorylase, brain form
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