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AB153607

Recombinant Human GPCR TGR5 protein (GST tag N-Terminus)

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Recombinant Human GPCR TGR5 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 330 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

TGR5, GPBAR1, G-protein coupled bile acid receptor 1, G-protein coupled receptor GPCR19, Membrane-type receptor for bile acids, hBG37, hGPCR19, M-BAR, BG37

1 Images
SDS-PAGE - Recombinant Human GPCR TGR5 protein (GST tag N-Terminus) (AB153607)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GPCR TGR5 protein (GST tag N-Terminus) (AB153607)

ab153607 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q8TDU6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB72608

Anti-GPCR TGR5 antibody

BOND RX™ Validated

Western blot - Anti-GPCR TGR5 antibody (AB72608)

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Biochemicals

AB142091

TGR5 Receptor Agonist, TGR5 G-protein coupled receptor agonist

Chemical Structure - TGR5 Receptor Agonist, TGR5 G-protein coupled receptor agonist (AB142091)

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Proteins & Peptides

AB235719

Recombinant Human GLP-1 protein (Tagged)

SDS-PAGE - Recombinant Human GLP-1 protein (Tagged) (AB235719)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MTPNSTGEVPSPIPKGALGLSLALASLIITANLLLALGIAWDRRLRSPPAGCFFLSLLLAGLLTGLALPTLPGLWNQSRRGYWSCLLVYLAPNFSFLSLLANLLLVHGERYMAVLRPLQPPGSIRLALLLTWAGPLLFASLPALGWNHWTPGANCSSQAIFPAPYLYLEVYGLLLPAVGAAAFLSVRVLATAHRQLQDICRLERAVCRDEPSALARALTWRQARAQAGAMLLFGLCWGPYVATLLLSVLAYEQRPPLGPGTLLSLLSLGSASAAAVPVAMGLGDQRYTAPWRAAAQRCLQGLWGRASRDSPGPSIAYHPSSQSSVDLDLN","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":330,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8TDU6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPCR TGR5 also known as G protein-coupled bile acid receptor 1 (GPBAR1) is a member of the GPCR superfamily. This receptor weighs approximately 35 kDa and is expressed in various tissues including liver intestine brown adipose tissue and macrophages. It functions as a membrane receptor that binds bile acids which activates downstream signaling pathways influencing metabolic processes.
Biological function summary

GPCR TGR5 plays a role in energy metabolism and the immune response. It does not form part of a larger receptor complex but interacts with different proteins to mediate its effects. Upon activation by bile acids TGR5 stimulates the production of cyclic AMP (cAMP) and regulates the release of various cytokines influencing metabolic rate and promoting anti-inflammatory effects.

Pathways

GPCR TGR5 links to the cAMP signaling pathway and plays a role in the brown adipose tissue activation pathway. It is closely related to proteins like adenylate cyclase which it activates to increase cAMP levels and potentially interacts with nuclear receptors modifying gene expression linked to energy homeostasis and inflammation control.

GPCR TGR5 associates with metabolic disorders such as obesity and type 2 diabetes. Its role in regulating energy expenditure makes it a target for combating obesity where it influences pathways involving protein partners like adenylate cyclase. Additionally its involvement in controlling inflammation connects it to diseases involving immune dysregulation offering potential pathways for therapeutic interventions.
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Specifications

Form

Liquid

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General info

Function

G protein-coupled receptor for bile acid (PubMed : 12419312, PubMed : 12524422, PubMed : 32698187, PubMed : 32747649, PubMed : 35858343). Bile acid-binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 12419312, PubMed : 12524422, PubMed : 32698187, PubMed : 32747649, PubMed : 35858343). GPBAR1 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity (PubMed : 12419312, PubMed : 12524422, PubMed : 32698187, PubMed : 32747649, PubMed : 35858343). Activated by bile acids, such as lithocholate, deoxycholate, chenodeoxycholate and cholate, in descending order (PubMed : 12524422, PubMed : 32698187). Apart from their role in lipid dietary absorption and cholesterol catabolism, bile acids act as an important signaling molecule, involved in processes, such as energy expenditure or tissue inflammation (PubMed : 26541439). GPBAR1-mediated signaling promotes energy expenditure and adiposity reduction in brown adipose tissue by activating adenylate cyclase, leading to DIO2 activation (By similarity). Involved in bile acid promoted GLP-1 secretion (By similarity).

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

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Product protocols

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Target data

G protein-coupled receptor for bile acid (PubMed : 12419312, PubMed : 12524422, PubMed : 32698187, PubMed : 32747649, PubMed : 35858343). Bile acid-binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 12419312, PubMed : 12524422, PubMed : 32698187, PubMed : 32747649, PubMed : 35858343). GPBAR1 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity (PubMed : 12419312, PubMed : 12524422, PubMed : 32698187, PubMed : 32747649, PubMed : 35858343). Activated by bile acids, such as lithocholate, deoxycholate, chenodeoxycholate and cholate, in descending order (PubMed : 12524422, PubMed : 32698187). Apart from their role in lipid dietary absorption and cholesterol catabolism, bile acids act as an important signaling molecule, involved in processes, such as energy expenditure or tissue inflammation (PubMed : 26541439). GPBAR1-mediated signaling promotes energy expenditure and adiposity reduction in brown adipose tissue by activating adenylate cyclase, leading to DIO2 activation (By similarity). Involved in bile acid promoted GLP-1 secretion (By similarity).
See full target information GPBAR1
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