Recombinant Human GPI-PLD protein is a Human Full Length protein, in the 1 to 176 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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- The Journal of biological chemistry 298:1021852022Ceramide levels in blood plasma correlate with major depressive disorder severity and its neutralization abrogates depressive behavior in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabian Schumacher,Michael J Edwards,Christiane Mühle,Alexander Carpinteiro,Greg C Wilson,Barbara Wilker,Matthias Soddemann,Simone Keitsch,Norbert Scherbaum,Bernhard W Müller,Undine E Lang,Christoph Linnemann,Burkhard Kleuser,Christian P Müller,Johannes Kornhuber,Erich GulbinsPubMed 35753355
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M S A F R L W P G L L I M L G S L C H R G S P C G L S T H I E I G H R A L E F L Q L H N G R V N Y R E L L L E H Q D A Y Q A G I V F P D C F Y P S I C K G G K F H D V S E S T H W T P F L N A S V H Y I R E N Y P L P W E K D T E K L V A F L F G I T S H M A A D V S W H S L G L E Q G F L R T M G A I D F H G S Y S E A H S A G D F G T V Y L H L L N F L V V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Recombinant protein. |
Target data
Function
This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
Alternative names
PIGPLD1, GPLD1, Phosphatidylinositol-glycan-specific phospholipase D, PI-G PLD, Glycoprotein phospholipase D, Glycosyl-phosphatidylinositol-specific phospholipase D, GPI-PLD, GPI-specific phospholipase D
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Recombinant Human GPI-PLD protein is a Human Full Length protein, in the 1 to 176 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P80108-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M S A F R L W P G L L I M L G S L C H R G S P C G L S T H I E I G H R A L E F L Q L H N G R V N Y R E L L L E H Q D A Y Q A G I V F P D C F Y P S I C K G G K F H D V S E S T H W T P F L N A S V H Y I R E N Y P L P W E K D T E K L V A F L F G I T S H M A A D V S W H S L G L E Q G F L R T M G A I D F H G S Y S E A H S A G D F G T V Y L H L L N F L V V
- Accession
- P80108
- Protein length
- Full Length
- Predicted molecular weight
- 45.47 kDa
- Amino acids
- 1 to 176
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
- Sequence similarities
Belongs to the GPLD1 family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as GPLD1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glycosylphosphatidylinositol specific phospholipase D1 (GPI-PLD) is an enzyme that hydrolyzes glycosylphosphatidylinositol (GPI)-anchored proteins. Also known as GPLD1 it acts by cleaving the inositol phosphate linkage. GPI-PLD possesses a molecular mass of about 110 kDa and is found extensively in the plasma signaling its secretion from the liver. In certain studies researchers occasionally refer to it by its involved function in detaching GPI proteins.
- Biological function summary
GPI-PLD participates in remodeling the cell surface by releasing certain GPI-anchored proteins. It impacts cellular events like signal transduction and cell adhesion. GPI-PLD functions independently not as part of a larger complex. Observation of GPI-PLD activities reveals involvement in regulating immune responses as well as interactions with other proteins on cell membranes. Its ability to influence GPI protein release affects how cells communicate and adapt in various biological contexts.
- Pathways
The enzyme GPI-PLD plays a role in pathways involving lipid metabolism and the immune system particularly complement system activation. GPI-PLD collaborates with proteins such as phospholipase C in lipid regulatory pathways. Its activity impacts the balance and turnover of lipids which affects the complement cascade by modulating membrane-bound proteins. Through these interactions GPI-PLD participates in refining how immune responses unfold affecting related biological processes.
- Associated diseases and disorders
Research shows that GPI-PLD has connections with conditions like atherosclerosis and diabetes. Changes in its enzymatic activity and expression levels impact lipid metabolism which ties to atherosclerosis development. In diabetes improper regulation of GPI-PLD can alter inflammatory and immune responses. The enzyme's activity further connects with lipoprotein interactions placing emphasis on its link with apolipoprotein AI indicating a broader influence in disorders related to lipid metabolism and inflammation.
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1 product image
SDS-PAGE - Recombinant Human GPI-PLD protein (ab116799)
12.5% SDS-PAGE showing ab116799 at approximately 45.47kDa.
Stained with Coomassie Blue.
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