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AB139792

Recombinant Human GPT2 protein

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Recombinant Human GPT2 protein is a Human Full Length protein, in the 1 to 523 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

AAT2, ALT2, GPT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2, GPT 2

1 Images
SDS-PAGE - Recombinant Human GPT2 protein (AB139792)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GPT2 protein (AB139792)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8TD30

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMQRAAALVRRGCGPRTPSSWGRSQSSAAAEASAVLKVRPERSRRERILTLESMNPQVKAVEYAVRGPIVLKAGEIELELQRGIKKPFTEVIRANIGDAQAMGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA","proteinLength":"Full Length","predictedMolecularWeight":"60.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":523,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8TD30","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GPT2 protein also known as Glutamyl-Prolyl-tRNA Synthetase 2 is an enzyme essential for the translation process in protein biosynthesis. It functions by charging tRNA molecules with their respective amino acids specifically glutamic acid and proline. The GPT2 protein has a mass of approximately 170 kDa and is expressed in various tissues including muscles and kidneys. This widespread expression suggests its significant role in maintaining protein synthesis in different cellular environments.
Biological function summary

GPT2 enzyme plays an important role in catalyzing the attachment of amino acids to their corresponding tRNA molecules a vital step in ribosomal protein synthesis. As part of the aminoacyl-tRNA synthetases GPT2 forms a complex with other synthetases to ensure accurate decoding of mRNA during translation. These complexes are essential for preserving translational fidelity across diverse biological systems impacting numerous cellular functions.

Pathways

The GPT2 enzyme integrates into the translation pathway where it ensures efficient and precise protein synthesis. Its activity connects closely with the mTOR signaling pathway which regulates cell growth and metabolism in response to nutritional status. GPT2 interacts with other tRNA synthetases and translation initiation factors forming a critical component of the protein synthesis mechanism. This highlights its coordination with other proteins to uphold proper cellular function.

GPT2 has associations with certain mitochondrial diseases due to defects in protein synthesis and metabolism. Errors or mutations in tRNA synthetases including GPT2 can lead to mitochondrial dysfunction and related disorders such as Charcot-Marie-Tooth disease. This connection implicates GPT2 and other proteins in its group like glycyl-tRNA synthetase in these neuropathological conditions. Understanding these associations can facilitate the development of targeted therapies for related diseases.
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Specifications

Form

Liquid

Additional notes

ab139792 is purified using conventional chromatography techniques.

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General info

Function

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.

Sequence similarities

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.
See full target information GPT2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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