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AB103052

Recombinant Human GRAP2 protein

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Recombinant Human GRAP2 protein is a Human Full Length protein, in the 1 to 330 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

GADS, GRB2L, GRID, GRAP2, GRB2-related adapter protein 2, Adapter protein GRID, GRB-2-like protein, GRBLG, GRBX, Grf40 adapter protein, Growth factor receptor-binding protein, Hematopoietic cell-associated adapter protein GrpL, P38, Protein GADS, SH3-SH2-SH3 adapter Mona, Grf-40

1 Images
SDS-PAGE - Recombinant Human GRAP2 protein (AB103052)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human GRAP2 protein (AB103052)

15% SDS-PAGE analysis of ab103052 (3μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O75791

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0292% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMEAVAKFDFTASGEDELSFHTGDVLKILSNQEEWFKAELGSQEGYVPKNFIDIQFPKWFHEGLSRHQAENLLMGKEVGFFIIRASQSSPGDFSISVRHEDDVQHFKVMRDNKGNYFLWTEKFPSLNKLVDYYRTNSISRQKQIFLRDRTREDQGHRGNSLDRRSQGGPHLSGAVGEEIRPSMNRKLSDHPPTLPLQQHQHQPQPPQYAPAPQQLQQPPQQRYLQHHHFHQERRGGSLDINDGHCGTGLGSEMNAALMHRRHTDPVQLQAAGRVRWARALYDFEALEDDELGFHSGEVVEVLDSSNPSWWTGRLHNKLGLFPANYVAPMTR","proteinLength":"Full Length","predictedMolecularWeight":"40 kDa","actualMolecularWeight":null,"aminoAcidEnd":330,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O75791","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GRAP2 also known as Gads plays an important role in intracellular signaling mechanisms. It is an adaptor protein with a mass of about 37 kDa. GRAP2 predominantly expresses in hematopoietic cells where it facilitates specific downstream signaling pathways. The protein assists in linking cell surface receptors to downstream signaling molecules enabling effective transmission of cellular signals.
Biological function summary

GRAP2 participates in the immune response by acting as a mediator that aids the assembly of signaling complexes. It often interacts with other proteins such as SLP-76 which is involved in specific immune cell activation. GRAP2 forms part of larger signaling complexes which are critical for the proper development and function of immune cells. This role highlights its importance in maintaining efficient immune surveillance and response.

Pathways

GRAP2 significantly contributes to the T-cell receptor (TCR) signaling pathway. It plays an integral part by interacting with proteins like LAT and ZAP-70 providing a scaffold for the further propagation of activation signals. Additionally in the Ras signaling pathway GRAP2 associates with other adaptor proteins coordinating downstream signaling needed for T cell activation and function.

GRAP2 has associations with immune system Pact conditions notably autoimmune diseases such as systemic lupus erythematosus (SLE). Its interactions with proteins like LAT and SLP-76 are important in these dysregulation of immune responses seen in these disorders. Any alteration in GRAP2 function or expression pathways could therefore potentially contribute to pathological immune activation reflecting its potential as a therapeutic target.
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Specifications

Form

Liquid

Additional notes

ab103052 was purified using conventional chromatography techniques.

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General info

Function

Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.

Sequence similarities

Belongs to the GRB2/sem-5/DRK family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.
See full target information GRAP2
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