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AB127666

Recombinant Human GRB 14 protein (His-DHFR tag N-Terminus)

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Recombinant Human GRB 14 protein (His-DHFR tag N-Terminus) is a Human Fragment protein, in the 75 to 249 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

Growth factor receptor-bound protein 14, GRB14 adapter protein, GRB14

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q14449

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"20.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":249,"aminoAcidStart":75,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q14449","tags":[{"tag":"His-DHFR","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRB14 also known as GRB (growth factor receptor-bound protein 14) is a member of GRB protein family. The mass of GRB14 is approximately 60 kDa. It is typically expressed in insulin-sensitive tissues such as the liver adipose tissue and skeletal muscle. Mechanically GRB14 acts as an adaptor protein binding to receptor tyrosine kinases modulating their signaling pathways and affecting downstream cellular responses.
Biological function summary

One of GRB14's main roles is negative regulation of insulin signaling. It is part of a protein complex that includes the insulin receptor where it inhibits insulin receptor kinase activity. This regulation impacts cellular glucose uptake and metabolism. GRB14 plays a critical role in controlling cell growth differentiation and survival due to its regulatory function in these pathways.

Pathways

GRB14 is involved extensively in the insulin and IGF (Insulin-like Growth Factor) receptor signaling pathways. It interacts closely with proteins such as the insulin receptor and IGF1 receptor. This interaction with receptor kinases positions GRB14 as a modulator of metabolic pathways influencing cellular energy balance and nutrient regulation.

GRB14 has a significant connection to metabolic diseases such as type 2 diabetes. Its interaction with the insulin receptor relates to insulin resistance an important feature of this disorder. Additionally GRB14's dysregulation might contribute to cancer progression due to its role in cell signaling pathways that control cell proliferation. These connections make GRB14 a target of interest in studying metabolic disease and oncogenesis.
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Specifications

Form

Lyophilized

Additional notes

Purified via His tag

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General info

Function

Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.

Sequence similarities

Belongs to the GRB7/10/14 family.

Post-translational modifications

Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2.

Subcellular localisation

Endosome membrane

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Product protocols

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Target data

Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.
See full target information Growth factor receptor-bound protein 14
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Product promise

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