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AB276653

Recombinant Human GRIK2/GluK2 protein (Fc Chimera)

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Recombinant Human GRIK2/GluK2 protein (Fc Chimera) is a Human Fragment protein, in the 1 to 561 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

GLUR6, GRIK2, GluK2, Excitatory amino acid receptor 4, Glutamate receptor 6, EAA4, GluR-6, GluR6

1 Images
SDS-PAGE - Recombinant Human GRIK2/GluK2 protein (Fc Chimera) (AB276653)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GRIK2/GluK2 protein (Fc Chimera) (AB276653)

SDS-PAGE analysis of ab276653

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q13002

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 100% PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MKIIFPILSNPVFRRTVKLLLCLLWIGYSQGTTHVLRFGGIFEYVESGPMGAEELAFRFAVNTINRNRTLLPNTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSDNKDSFYVSLYPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQLPADTKDAKPLLKEMKRGKEFHVIFDCSHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMTGFRILNTENTQVSSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQMTVSSLQCNRHKPWRFGTRFMSLIKEAHWEGLTGRITFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPASGLNMTESQKGKPANITDSLSNRSLIVTTILEEPYVLFKKSDKPLYGNDRFEGYCIDLLRELSTILGFTYEIRLVEDGKYGAQDDANGQWNGMVRELIDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSP","proteinLength":"Fragment","predictedMolecularWeight":"86.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":561,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q13002","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GRIK2 protein also known as GluK2 is a subunit of the kainate receptor which is part of the ionotropic glutamate receptor family. This receptor primarily mediates excitatory synaptic transmission in the central nervous system. GRIK2 has a molecular weight of approximately 104 kDa and is highly expressed in regions such as the hippocampus cortex and cerebellum. These areas are critical for learning and memory processes which underlines the importance of GluK2 in neuronal communication.
Biological function summary

GRIK2 plays a significant role in modulating synaptic plasticity an important mechanism for learning and memory. It forms heteromeric complexes with other kainate receptor subunits like GluK1 and GluK3 which can affect receptor properties. This combination allows for diverse functional responses and adaptability in synaptic transmission. The presence of GRIK2 in neurons indicates that it is integral in processes such as synaptic strength modulation and response to different synaptic inputs.

Pathways

GRIK2 is essential in the glutamatergic signaling pathway which is involved in many excitatory transmissions within the brain. This protein interacts with associated proteins such as PSD-95 and PICK1 which helps in receptor clustering and synaptic signaling regulation. Additionally GRIK2 is involved in calcium signaling pathways through its ability to regulate calcium influx contributing to neuron function and communication.

GRIK2 has been linked to conditions such as epilepsy and schizophrenia. Mutations or dysregulation in the expression of GRIK2 can affect neurotransmission balance which can lead to these neurological disorders. Its interaction with other receptor subunits like GluK1 and GluK3 also connects GRIK2 to these diseases since alterations in the complex can disrupt normal synaptic function. Understanding these relationships is key in developing therapeutic strategies for such disorders.
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Specifications

Form

Lyophilized

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General info

Function

Ionotropic glutamate receptor that functions as a cation permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (PubMed : 14511640, PubMed : 28180184, PubMed : 34375587, PubMed : 7536611, PubMed : 8730589). Modulates cell surface expression of NETO2. In association with GRIK3, involved in presynaptic facilitation of glutamate release at hippocampal mossy fiber synapses (By similarity).. Independent of its ionotropic glutamate receptor activity, acts as a thermoreceptor conferring sensitivity to cold temperatures (PubMed : 31474366). Functions in dorsal root ganglion neurons (By similarity).

Sequence similarities

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily.

Post-translational modifications

Sumoylation mediates kainate receptor-mediated endocytosis and regulates synaptic transmission. Sumoylation is enhanced by PIAS3 and desumoylated by SENP1 (By similarity).. Ubiquitinated. Ubiquitination regulates the GRIK2 levels at the synapse by leading kainate receptor degradation through proteasome (By similarity).. Phosphorylated by PKC at Ser-868 upon agonist activation, this directly enhance sumoylation.

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Product protocols

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Target data

Ionotropic glutamate receptor that functions as a cation permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (PubMed : 14511640, PubMed : 28180184, PubMed : 34375587, PubMed : 7536611, PubMed : 8730589). Modulates cell surface expression of NETO2. In association with GRIK3, involved in presynaptic facilitation of glutamate release at hippocampal mossy fiber synapses (By similarity).. Independent of its ionotropic glutamate receptor activity, acts as a thermoreceptor conferring sensitivity to cold temperatures (PubMed : 31474366). Functions in dorsal root ganglion neurons (By similarity).
See full target information GRIK2
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