Recombinant human GRK1 protein
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Recombinant human GRK1 protein is a Human Full Length protein, in the 1 to 563 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for WB, FuncS.
View Alternative Names
RHOK, GRK1, Rhodopsin kinase GRK1, RK, G protein-coupled receptor kinase 1
- FuncS
Unknown
Functional Studies - Recombinant human GRK1 protein (AB105126)
Kinase Activity Plot. Specific activity is 4.3 nmol/min/mg
- WB
Unknown
Western blot - Recombinant human GRK1 protein (AB105126)
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Western blot - Recombinant human GRK1 protein (ab105126)
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Reactivity data
Product details
It is recommended to store this protein at -80°C in glycerol to preserve ts activity.
ab204870 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GRK1 functions as part of a coordinated mechanism that ensures proper desensitization and recovery of photoreceptors after they are exposed to light. It is instrumental in the phototransduction cascade preventing excessive activation by phosphorylating light-activated rhodopsin which then allows the binding of arrestin. This phosphorylation-arrestin binding step is part of a larger complex that maintains the sensitivity of photoreceptors to changing light conditions.
Pathways
GRK1 participates in the critical visual signal transduction pathway within the retina. It works in conjunction with proteins such as arrestin and rhodopsin to modulate the phototransduction cascade ensuring proper visual responses. This kinase interacts with these proteins to modulate their activity and help in adapting to varying light conditions emphasizing its importance in maintaining visual clarity.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed : 15946941). This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity). May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Post-translational modifications
Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.. Farnesylation is required for full activity.
Target data
Complementary Products
Product promise
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