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AB125620

Recombinant human GRK2 protein

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Recombinant human GRK2 protein is a Human Full Length protein, in the 2 to 689 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

ADRBK1, BARK, BARK1, GRK2, Beta-adrenergic receptor kinase 1, Beta-ARK-1, G-protein coupled receptor kinase 2

4 Images
Functional Studies - Recombinant human GRK2 protein (AB125620)
  • FuncS

Unknown

Functional Studies - Recombinant human GRK2 protein (AB125620)

The specific activity of ab125620 was determined to be 5.3 nmol/mg/min by Kinase Assay.

Functional Studies - Recombinant human GRK2 protein (AB125620)
  • FuncS

Supplier Data

Functional Studies - Recombinant human GRK2 protein (AB125620)

The specific activity of GRK2 (ab125620) was determined to be 5 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human GRK2 protein (AB125620)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human GRK2 protein (AB125620)

SDS-PAGE analysis of ab125620.

SDS-PAGE - Recombinant human GRK2 protein (AB125620)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human GRK2 protein (AB125620)

SDS PAGE analysis of ab125620

Key facts

Purity

>90% Densitometry

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab125620 was determined to be 5.3 nmol/mg/min.

Accession

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Complementary Products

Reagents, Controls & Accessories

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Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

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Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

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Primary Antibodies

AB218624

Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

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Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [EPR21801] - Early Endosome Marker (AB218624)

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Proteins & Peptides

AB132072

Recombinant Human S1P1/EDG1 protein (GST tag N-Terminus)

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Primary Antibodies

AB227825

Anti-GRK2 antibody [EPR22465]

RabMAb|Recombinant|KO Validated|Trial Size

Immunoprecipitation - Anti-GRK2 antibody [EPR22465] (AB227825)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204876 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"102 kDa","actualMolecularWeight":null,"aminoAcidEnd":689,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P25098","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GRK2 also known as G protein-coupled receptor kinase 2 is an important enzyme involved in the regulation of G protein-coupled receptors (GPCRs). It has a molecular weight of approximately 80 kilodaltons. GRK2 is widely expressed in many tissues especially in the heart and brain where it plays an important role in cellular signaling processes. Its primary action is phosphorylating activated GPCRs which leads to receptor desensitization and downregulation modulating cellular responses to stimuli.
Biological function summary

GRK2 acts by coordinating with other proteins to regulate signal transduction pathways. It usually forms a complex with arrestins which prevent further signaling by blocking G protein coupling and directing receptors towards internalization pathways. This kinase acts to modulate receptor activity and maintain cellular homeostasis. GRK2 is essential for preventing overstimulation of cells playing a role in neurobiology and cardiology.

Pathways

GRK2 plays an important role in the adrenergic receptor signaling pathways including the beta-adrenergic and alpha-adrenergic pathways. It participates in the regulation of cardiac output and vascular tone by influencing the response of cells to catecholamines like adrenaline and noradrenaline. Proteins related to GRK2 in these pathways include adrenergic receptor subtypes and arrestin proteins. Voltage-gated calcium channels also interact with GRK2 linking it to calcium signaling pathways vital for muscle contraction and neurotransmitter release.

Research highlights GRK2's involvement in heart failure and hypertension. In heart failure elevated levels of GRK2 correlate with worsened cardiac function due to impaired beta-adrenergic receptor signaling. GRK2 is also linked to the development of hypertension through its effects on vascular smooth muscle cells and blood pressure regulation. Its interaction with proteins like arrestins suggests potential therapeutic targets for these cardiovascular conditions. Understanding and modulating GRK2 activity might offer approaches to treat these disorders.
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General info

Function

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them (PubMed : 19715378). Phosphorylates catecholamine-activated ADRB2 to regulate physiological cardiomyocyte contraction rate responses (By similarity). Also phosphorylates ligand-bound C3a and C5a anaphylatoxin receptors (C3AR1 and C5AR1, respectively), leading to receptor desensitization (PubMed : 21799898, PubMed : 23077507). Key regulator of LPAR1 signaling (PubMed : 19306925). Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor (PubMed : 19306925). Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner (PubMed : 19306925). Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity). Inhibits relaxation of airway smooth muscle in response to blue light (PubMed : 30284927).

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

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Product protocols

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Target data

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them (PubMed : 19715378). Phosphorylates catecholamine-activated ADRB2 to regulate physiological cardiomyocyte contraction rate responses (By similarity). Also phosphorylates ligand-bound C3a and C5a anaphylatoxin receptors (C3AR1 and C5AR1, respectively), leading to receptor desensitization (PubMed : 21799898, PubMed : 23077507). Key regulator of LPAR1 signaling (PubMed : 19306925). Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor (PubMed : 19306925). Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner (PubMed : 19306925). Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity). Inhibits relaxation of airway smooth muscle in response to blue light (PubMed : 30284927).
See full target information GRK2
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