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AB268605

Recombinant Human GRK4 protein (Tagged)

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Recombinant Human GRK4 protein (Tagged) is a Human Fragment protein, in the 140 to 578 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE.

View Alternative Names

GPRK2L, GPRK4, GRK4, G protein-coupled receptor kinase 4, G protein-coupled receptor kinase GRK4, ITI1

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SDS-PAGE - Recombinant Human GRK4 protein (Tagged) (AB268605)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GRK4 protein (Tagged) (AB268605)

SDS-PAGE analysis of ab268605.

MW ~75 kDa.

Key facts

Purity

>70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P32298

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"KAFEECTRVAHNYLRGEPFEEYQESSYFSQFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLQKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLGNPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTVGYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSICRMLLTKNPSKRLGCRGEGAAGVKQHPVFKDINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSVVKGIYLDTADEDFYARFATGCVSIPWQNEMIESGCFKDINKSESEEALPLDLDKNIHTPVSRPNRGFFYRLFRRGGCLTMVPSEKEVEPKQC","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":578,"aminoAcidStart":140,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P32298","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GRK4 or G protein-coupled receptor kinase 4 belongs to the family of serine/threonine kinases. It plays a mechanical role in phosphorylating activated G protein-coupled receptors (GPCRs) which leads to receptor desensitization. GRK4 has a molecular mass of about 66 kDa and is widely expressed in tissues including the kidney testis and brain. Such expression pattern suggests its involvement in numerous physiological processes.
Biological function summary

G protein-coupled receptor kinase 4 regulates GPCR signaling by facilitating receptor internalization and degradation. This action modulates cellular responsiveness to hormones and neurotransmitters. GRK4 does not form part of a complex but its regulatory function directly impacts GPCR pathways. GRK4 achieves this control by adding phosphate groups typically functioning as a feedback mechanism during receptor activity.

Pathways

GRK4 serves as an important regulator in the MAPK signaling pathway and the renin-angiotensin-aldosterone system (RAAS). In MAPK pathways GRK4 helps mediate signal transduction by controlling receptor activity. It also interacts with other GRKs like GRK2 to fine-tune cellular responses and maintain homeostasis. Its involvement in RAAS highlights its significance in cardiovascular regulation.

G protein-coupled receptor kinase 4 has strong links to hypertension and Parkinson's disease. Its dysregulation or genetic variants can lead to abnormal blood pressure regulation by altering dopamine receptors via RAAS. In Parkinson's disease GRK4's influence on dopamine-related pathways suggests a connection to the condition's neurological symptoms. Aberrations in GRK4 activities may also impact interactions with proteins like GRK6 contributing to disease progression.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Post-translational modifications

Palmitoylated.

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Product protocols

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Target data

Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.
See full target information GRK4
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