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AB92858

Recombinant Human GRO gamma protein (MBP)

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(1 Publication)

Recombinant Human GRO gamma protein (MBP) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, ELISA, Mass Spec, WB.

View Alternative Names

GRO3, GROG, SCYB3, CXCL3, C-X-C motif chemokine 3, GRO-gamma(1-73), Growth-regulated protein gamma, Macrophage inflammatory protein 2-beta, GRO-gamma, MIP2-beta

2 Images
Western blot - Recombinant Human GRO gamma protein (AB92858)
  • WB

Unknown

Western blot - Recombinant Human GRO gamma protein (AB92858)

All lanes:

Anti-GRO gamma antibody (<a href='/en-us/products/unavailable/gro-gamma-antibody-ab9843'>ab9843</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human GRO gamma protein (ab92858) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 10s

SDS-PAGE - Recombinant Human GRO gamma protein (AB92858)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GRO gamma protein (AB92858)

10% SDS-PAGE of ab92858 stained with Coomassie Blue (CB) and immunoblotted with anti-MBP (WB).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

MBP tag

Applications

ELISA, Mass Spec, WB, SDS-PAGE

applications

Biologically active

No

Accession

P19876

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 30% Glycerol (glycerin, glycerine)

storage-buffer

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Primary Antibodies

AB220431

Anti-GRO gamma antibody [EPR21759-11]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-GRO gamma antibody [EPR21759-11] (AB220431)

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Assay Kits

AB234574

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ELISA - Human CXCL3 ELISA Kit (AB234574)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab92858 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/gro-gamma-antibody-ab9843'>ab9843</a>.</p>" } } }
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P19876","tags":[{"tag":"MBP","terminus":""}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRO gamma also known as CXCL3 is a chemokine with a molecular weight of approximately 7.9 kDa. It is a small protein belonging to the CXC chemokine family. GRO gamma is expressed in numerous tissues including epithelial cells fibroblasts and several leukocytes. This protein plays a significant role in chemotactic signaling drawing neutrophils to sites of inflammation and infection acting as both a signal and effector molecule in immune response. Researchers often use CXCL3 ELISA kits and GRO protein assays to quantify its expression levels in biological samples.
Biological function summary

GRO gamma functions as a chemokine that guides neutrophils by binding to its receptor CXCR2. It participates in inflammatory processes and is produced in response to stimuli such as bacterial products and pro-inflammatory cytokines. GRO gamma together with its close family members forms a network facilitating the immune cell recruitment and activation at infection sites. Also this chemokine is included in larger chemokine complexes influencing not just immune cells but also modulating angiogenesis and cellular matrix restructuring.

Pathways

GRO gamma significantly contributes to the chemokine signaling pathway and the NF-kB signaling pathway. In these pathways it interacts tightly with proteins like CXCR2 which is essential for the signaling cascade that results in immune cell migration. It also engages with the MAPK pathways impacting cell proliferation and apoptosis. These involvements make GRO gamma vital for orchestrating the inflammation response in humans.

GRO gamma is linked to chronic inflammatory conditions and cancer. Elevated levels of GRO gamma have been observed in rheumatoid arthritis where its interaction with CXCR2 exacerbates inflammation and tissue damage. In cancer particularly in colorectal cancer GRO gamma plays a role in tumor progression by promoting angiogenesis and cell proliferation interacting with VEGF and other cytokines. Understanding its functions and relationships opens potential avenues for therapeutic interventions targeting chronic diseases and cancer.
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Specifications

Form

Liquid

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General info

Function

Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.

Sequence similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Post-translational modifications

N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.

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Product protocols

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Target data

Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
See full target information CXCL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology reports 47:5927-5942 PubMed32691273

2020

Role of the O-GlcNAc modification on insulin resistance and endoplasmic reticulum stress in 3T3-L1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Benan Pelin Sermikli,Gulizar Aydogdu,Erkan Yilmaz
View all publications
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Product promise

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