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Recombinant human Growth Hormone protein (Active) is a Human Full Length protein, in the 27 to 217 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, WB.

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Images

SDS-PAGE - Recombinant human Growth Hormone protein (Active) (AB51232), expandable thumbnail
  • Western blot - Recombinant human Growth Hormone protein (Active) (AB51232), expandable thumbnail

Publications

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
Escherichia coli
Tags
Tag free
Applications
SDS-PAGE, FuncS, WB
Biologically active
Yes

Amino acid sequence

F P T I P L S R L F D N A M L R A H R L H Q L A F D T Y Q E F E E A Y I P K E Q K Y S F L Q N P Q T S L C F S E S I P T P S N R E E T Q Q K S N L E L L R I S L L L I Q S W L E P V Q F L R S V F A N S L V Y G A S D S N V Y D L L K D L E E G I Q T L M G R L E D G S P R T G Q I F K Q T Y S K F D T N S H N D D A L L K N Y G L L Y C F R K D M D K V E T F L R I V Q C R S V E G S C G F

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

ab51232 can be used as a WB positive control in conjunction with Anti-Growth Hormone antibody [GH-1] ab9821.

Associated Products

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3 products for Alternative Product

Target data

Function

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Alternative names

Recommended products

Recombinant human Growth Hormone protein (Active) is a Human Full Length protein, in the 27 to 217 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, WB.

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
Escherichia coli
Applications
SDS-PAGE, FuncS, WB
Biological activity
Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.2ng/ml.
Accession
P01241-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 1.6016% Mannitol, 0.0134% PBS

Sequence info

Amino acid sequence

F P T I P L S R L F D N A M L R A H R L H Q L A F D T Y Q E F E E A Y I P K E Q K Y S F L Q N P Q T S L C F S E S I P T P S N R E E T Q Q K S N L E L L R I S L L L I Q S W L E P V Q F L R S V F A N S L V Y G A S D S N V Y D L L K D L E E G I Q T L M G R L E D G S P R T G Q I F K Q T Y S K F D T N S H N D D A L L K N Y G L L Y C F R K D M D K V E T F L R I V Q C R S V E G S C G F
Accession
P01241
Protein length
Full Length
Predicted molecular weight
22.1 kDa
Amino acids
27 to 217
Nature
Recombinant

Specifications

Form
Liquid
Additional notes

Recombinant human Growth Hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

General info

Function

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Sequence similarities

Belongs to the somatotropin/prolactin family.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Growth Hormone (GH) also known as somatotropin is a polypeptide hormone with a mass of approximately 22 kDa. The pituitary gland synthesizes and secretes GH. Once synthesized GH circulates in the bloodstream and affects various tissues throughout the body. GH's expression is tightly regulated because of its significant roles in growth processes. Researchers might use anti-growth hormone antibodies and various hGH testing kits including ELISA to study its levels and functions.

Biological function summary

GH triggers growth and cell reproduction by exerting effects on multiple tissues. It binds to the growth hormone receptor which initiates a cascade of reactions that ultimately stimulates growth in muscle bone and cartilage. GH also contributes to protein synthesis and helps regulate metabolism. GH acts mostly independently but can interact with cytokines and other hormones.

Pathways

GH plays important roles in the regulation of the JAK-STAT signaling pathway and the IGF-1 signaling pathway. The JAK-STAT pathway is important for transmitting the signal of GH binding to its receptor into cellular responses. GH stimulates IGF-1 production in the liver and IGF-1 then acts in an endocrine manner to mediate much of GH’s growth-promoting activity. These interactions highlight the interconnectedness of GH with other hormones in growth signaling networks.

Associated diseases and disorders

Several health conditions can arise from abnormal levels of GH. Dwarfism occurs due to GH deficiency while acromegaly results from GH excess. Clinical analysis might use hGH kits to measure hormone levels and aid in diagnosis. GH's role in such conditions links it to other hormones like insulin and glucagon that help regulate glucose levels further affecting metabolic processes. Recombinant forms of GH are used in therapy for these disorders providing avenues for treatment and management.

Product promise

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2 product images

  • SDS-PAGE - Recombinant human Growth Hormone protein (Active) (ab51232), expandable thumbnail

    SDS-PAGE - Recombinant human Growth Hormone protein (Active) (ab51232)

    15% SDS-PAGE - Recombinant human Growth Hormone protein (Active) (ab51232, 3 μg).

    Reducing conditions. Visualized by coomassie blue stain.

  • Western blot - Recombinant human Growth Hormone protein (Active) (ab51232), expandable thumbnail

    Western blot - Recombinant human Growth Hormone protein (Active) (ab51232)

    All lanes: Western blot - Anti-Growth Hormone antibody [GH-1] (Anti-Growth Hormone antibody [GH-1] ab9821) at 1/500 dilution

    Lane 1: Western blot - Recombinant human Growth Hormone protein (Active) (ab51232) at 0.1 µg

    Lane 2: Western blot - Recombinant human Growth Hormone protein (Active) (ab51232) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa

    Exposure time: 2min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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