Recombinant human Growth Hormone protein (Active) is a Human Full Length protein, in the 27 to 217 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, WB.
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- PloS one 10:e01347232015Enabling Low Cost Biopharmaceuticals: A Systematic Approach to Delete Proteases from a Well-Known Protein Production Host Trichoderma reesei.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher P Landowski et. al.PubMed 26309247
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, WB
- Biologically active
- Yes
Amino acid sequence
F P T I P L S R L F D N A M L R A H R L H Q L A F D T Y Q E F E E A Y I P K E Q K Y S F L Q N P Q T S L C F S E S I P T P S N R E E T Q Q K S N L E L L R I S L L L I Q S W L E P V Q F L R S V F A N S L V Y G A S D S N V Y D L L K D L E E G I Q T L M G R L E D G S P R T G Q I F K Q T Y S K F D T N S H N D D A L L K N Y G L L Y C F R K D M D K V E T F L R I V Q C R S V E G S C G F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab51232 can be used as a WB positive control in conjunction with Anti-Growth Hormone antibody [GH-1] ab9821. |
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Target data
Function
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Alternative names
Somatotropin, Growth hormone, Growth hormone 1, Pituitary growth hormone, GH, GH-N, GH1
Recommended products
Recombinant human Growth Hormone protein (Active) is a Human Full Length protein, in the 27 to 217 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, WB
- Biologically active
- Yes
- Biological activity
- Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.2ng/ml.
- Accession
- P01241-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 1.6016% Mannitol, 0.0134% PBS
Sequence info
Amino acid sequence
- F P T I P L S R L F D N A M L R A H R L H Q L A F D T Y Q E F E E A Y I P K E Q K Y S F L Q N P Q T S L C F S E S I P T P S N R E E T Q Q K S N L E L L R I S L L L I Q S W L E P V Q F L R S V F A N S L V Y G A S D S N V Y D L L K D L E E G I Q T L M G R L E D G S P R T G Q I F K Q T Y S K F D T N S H N D D A L L K N Y G L L Y C F R K D M D K V E T F L R I V Q C R S V E G S C G F
- Accession
- P01241
- Protein length
- Full Length
- Predicted molecular weight
- 22.1 kDa
- Amino acids
- 27 to 217
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Recombinant human Growth Hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
General info
- Function
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
- Sequence similarities
Belongs to the somatotropin/prolactin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Growth Hormone (GH) also known as somatotropin is a polypeptide hormone with a mass of approximately 22 kDa. The pituitary gland synthesizes and secretes GH. Once synthesized GH circulates in the bloodstream and affects various tissues throughout the body. GH's expression is tightly regulated because of its significant roles in growth processes. Researchers might use anti-growth hormone antibodies and various hGH testing kits including ELISA to study its levels and functions.
- Biological function summary
GH triggers growth and cell reproduction by exerting effects on multiple tissues. It binds to the growth hormone receptor which initiates a cascade of reactions that ultimately stimulates growth in muscle bone and cartilage. GH also contributes to protein synthesis and helps regulate metabolism. GH acts mostly independently but can interact with cytokines and other hormones.
- Pathways
GH plays important roles in the regulation of the JAK-STAT signaling pathway and the IGF-1 signaling pathway. The JAK-STAT pathway is important for transmitting the signal of GH binding to its receptor into cellular responses. GH stimulates IGF-1 production in the liver and IGF-1 then acts in an endocrine manner to mediate much of GH’s growth-promoting activity. These interactions highlight the interconnectedness of GH with other hormones in growth signaling networks.
- Associated diseases and disorders
Several health conditions can arise from abnormal levels of GH. Dwarfism occurs due to GH deficiency while acromegaly results from GH excess. Clinical analysis might use hGH kits to measure hormone levels and aid in diagnosis. GH's role in such conditions links it to other hormones like insulin and glucagon that help regulate glucose levels further affecting metabolic processes. Recombinant forms of GH are used in therapy for these disorders providing avenues for treatment and management.
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2 product images
SDS-PAGE - Recombinant human Growth Hormone protein (Active) (ab51232)
15% SDS-PAGE - Recombinant human Growth Hormone protein (Active) (ab51232, 3 μg).
Reducing conditions. Visualized by coomassie blue stain.
Western blot - Recombinant human Growth Hormone protein (Active) (ab51232)
All lanes: Western blot - Anti-Growth Hormone antibody [GH-1] (Anti-Growth Hormone antibody [GH-1] ab9821) at 1/500 dilution
Lane 1: Western blot - Recombinant human Growth Hormone protein (Active) (ab51232) at 0.1 µg
Lane 2: Western blot - Recombinant human Growth Hormone protein (Active) (ab51232) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Exposure time: 2min
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