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Recombinant human Growth Hormone protein (Active) is a Human Full Length protein, in the 27 to 217 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, WB.
Alternative names=Somatotropin, Growth hormone, Growth hormone 1, Pituitary growth hormone, GH, GH-N, GH1
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, WB
- Biologically active
Yes
- Sequence
F P T I P L S R L F D N A M L R A H R L H Q L A F D T Y Q E F E E A Y I P K E Q K Y S F L Q N P Q T S L C F S E S I P T P S N R E E T Q Q K S N L E L L R I S L L L I Q S W L E P V Q F L R S V F A N S L V Y G A S D S N V Y D L L K D L E E G I Q T L M G R L E D G S P R T G Q I F K Q T Y S K F D T N S H N D D A L L K N Y G L L Y C F R K D M D K V E T F L R I V Q C R S V E G S C G F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab51232 can be used as a WB positive control in conjunction with ab9821. |
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Recombinant human Growth Hormone protein (Active) is a Human Full Length protein, in the 27 to 217 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, WB.
Alternative names=Somatotropin, Growth hormone, Growth hormone 1, Pituitary growth hormone, GH, GH-N, GH1
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, WB
- Biologically active
Yes
- Biological activity
Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.2ng/ml.
- Predicted molecular weight
22.1 kDa
- Amino acids
27 to 217
- Sequence
F P T I P L S R L F D N A M L R A H R L H Q L A F D T Y Q E F E E A Y I P K E Q K Y S F L Q N P Q T S L C F S E S I P T P S N R E E T Q Q K S N L E L L R I S L L L I Q S W L E P V Q F L R S V F A N S L V Y G A S D S N V Y D L L K D L E E G I Q T L M G R L E D G S P R T G Q I F K Q T Y S K F D T N S H N D D A L L K N Y G L L Y C F R K D M D K V E T F L R I V Q C R S V E G S C G F
- Accession
- P01241-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 1.6016% Mannitol, 0.0134% PBS
Specifications
- Form
Liquid
- Additional notes
Recombinant human Growth Hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
General info
- Function
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
- Sequence similarities
Belongs to the somatotropin/prolactin family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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