Recombinant human Growth hormone receptor protein is a Human Fragment protein, in the 27 to 264 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Images
Publications
Filter by
- PloS one 18:e02827412023Amino acid substitutions in human growth hormone affect secondary structure and receptor binding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrei Rajkovic,Sandesh Kanchugal,Eldar Abdurakhmanov,Rebecca Howard,Sebastian Wärmländer,Joseph Erwin,Hugo A Barrera Saldaña,Astrid Gräslund,Helena Danielson,Samuel Coulbourn FloresPubMed 36952491
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
A I L S R A P W S L Q S V N P G L K T N S S K E P K F T K C R S P E R E T F S C H W T D E V H H G T K N L G P I Q L F Y T R R N T Q E W T Q E W K E C P D Y V S A G E N S C Y F N S S F T S I W I P Y C I K L T S N G G T V D E K C F S V D E I V Q P D P P I A L N W T L L N V S L T G I H A D I Q V R W E A P R N A D I Q K G W M V L E Y E L Q Y K E V N E T K W K M M D P I L T T S V P V Y S L K V D K E Y E V R V R S K Q R N S G N Y G E F S E V L Y V T L P Q M S Q F T C E E D F Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth (PubMed:1549776, PubMed:2825030, PubMed:8943276). On ligand binding, couples to the JAK2/STAT5 pathway (PubMed:1549776, PubMed:15690087, PubMed:2825030, PubMed:8943276). Growth hormone-binding protein. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2. Up-regulates the production of the soluble Growth hormone-binding protein form (GHBP) and acts as a negative inhibitor of growth hormone signaling.
Alternative names
Growth hormone receptor, GH receptor, Somatotropin receptor, GHR
Recommended products
Recombinant human Growth hormone receptor protein is a Human Fragment protein, in the 27 to 264 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized Human GH, Tag Free at 2μg/mL (100 μL/well) can bind Human GHR, His Tag (ab180053) with a linear range of 1-8 ng/mL (QC tested).
- Accession
- P10912-1
- Animal free
- No
- Species
- Human
- Reconstitution
- It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing. To avoid surface adsorption loss and inactivation the reconstituted protein must not be aliquoted to less than 10 µg per vial.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Trehalose
Sequence info
Amino acid sequence
- A I L S R A P W S L Q S V N P G L K T N S S K E P K F T K C R S P E R E T F S C H W T D E V H H G T K N L G P I Q L F Y T R R N T Q E W T Q E W K E C P D Y V S A G E N S C Y F N S S F T S I W I P Y C I K L T S N G G T V D E K C F S V D E I V Q P D P P I A L N W T L L N V S L T G I H A D I Q V R W E A P R N A D I Q K G W M V L E Y E L Q Y K E V N E T K W K M M D P I L T T S V P V Y S L K V D K E Y E V R V R S K Q R N S G N Y G E F S E V L Y V T L P Q M S Q F T C E E D F Y
- Accession
- P10912
- Protein length
- Fragment
- Predicted molecular weight
- 28.5 kDa
- Amino acids
- 27 to 264
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Purified by Immobilized metal affinity chromatography.
General info
- Function
Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth (PubMed:1549776, PubMed:2825030, PubMed:8943276). On ligand binding, couples to the JAK2/STAT5 pathway (PubMed:1549776, PubMed:15690087, PubMed:2825030, PubMed:8943276).
- Sequence similarities
Belongs to the type I cytokine receptor family. Type 1 subfamily.
- Post-translational modifications
The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE (PubMed:11785980). Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17 (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Growth Hormone Receptor (GHR) also known as somatotropin receptor is a vital protein that mediates the effects of growth hormone (GH). GHR is a transmembrane receptor with a mass of approximately 70 kDa and is expressed in many tissues including liver muscle and adipose tissue. It plays an important role in growth regulation by binding to growth hormone which triggers signal transduction pathways involved in cellular proliferation and growth.
- Biological function summary
The growth hormone receptor facilitates the hormone's ability to promote growth and cell differentiation. The receptor when activated stimulates the JAK2/STAT5 signaling pathway a part of a larger GHR complex. This complex leads to gene transcription that stimulates growth and metabolism. The activation of GHR has significant effects on the cellular functions related to protein synthesis lipid metabolism and glucose metabolism.
- Pathways
The growth hormone receptor is an important component of the JAK-STAT signaling pathway and the mTOR signaling pathway. In these pathways GHR interacts with other proteins such as Janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5). These interactions play a critical role in translating the binding of growth hormone into cellular responses that lead to growth and metabolic regulation.
- Associated diseases and disorders
Disruptions or mutations in the growth hormone receptor can lead to conditions such as Laron syndrome and growth hormone insensitivity. These conditions often display symptoms of dwarfism and metabolic aberrations due to impaired signaling. GHR malfunction relates closely to growth hormone (GH) and anti-growth hormone treatments like somatropin used for managing growth deficiencies. The connection of GHR with related pathways and proteins like the IGF-1 (Insulin-like Growth Factor 1) underlines its role in metabolic diseases.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
SDS-PAGE - Recombinant human Growth hormone receptor protein (ab180053)
Human GHR (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
SDS-PAGE - Recombinant human Growth hormone receptor protein (ab180053)
SDS-PAGE analysis of ab180053 in reducing conditions. Gel stained overnight with Coomassie Blue. DTT-reduced protein migrates as 40-50 kDa due to glycosylation.
Functional Studies - Recombinant human Growth hormone receptor protein (ab180053)
Immobilized Human GH, Tag Free Recombinant Human Growth Hormone Protein ab223106 at 2 μg/mL (100 μL/well) can bind Human GHR, His Tag (ab180053) with a linear range of 1-8 ng/mL (QC tested).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com