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AB105621

Recombinant Human GSKIP protein

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Recombinant Human GSKIP protein is a Human Full Length protein, in the 1 to 139 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C14orf129, HSPC210, GSKIP, GSK3B-interacting protein, GSK3beta interaction protein

1 Images
SDS-PAGE - Recombinant Human GSKIP protein (AB105621)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GSKIP protein (AB105621)

15% SDS-PAGE analysis of ab105621 (3μg)

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9P0R6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.29% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as HSPC210.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMETDCNPMELSSMSGFEEGSELNGFEGTDMKDMRLEAEAVVNDVLFAVNNMFVSKSLRCADDVAYINVETKERNRYCLELTEAGLKVVGYAFDQVDDHLQTPYHETVYSLLDTLSPAYREAFGNALLQRLEALKRDGQS","proteinLength":"Full Length","predictedMolecularWeight":"17.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":139,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9P0R6","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GSKIP also known as GSK3β-interacting protein plays a role in the signaling pathways by acting as a scaffold for protein interactions. This protein has an approximate mass of 17 kDa. GSKIP is expressed in various tissues including the brain and heart suggesting its broad functional significance in different physiological contexts. Its ability to interact with Glycogen Synthase Kinase 3 (GSK3) and other proteins highlights its involvement in important cellular processes.
Biological function summary

The protein functions as a regulatory adapter anchoring key kinases to their target proteins. It interacts with GSK3β forming a stable complex that influences several signaling pathways. By modulating these interactions GSKIP plays a role in controlling cell growth differentiation and apoptosis. Its capacity to form complexes with other proteins enhances its functional versatility within the cell impacting numerous biological processes.

Pathways

GSKIP exerts a notable influence in the Wnt/β-catenin and Hedgehog signaling pathways. It interacts with GSK3β which is a critical component of these pathways regulating cellular processes like proliferation and development. By affecting the stability and activity of β-catenin GSKIP indirectly influences gene expression. Additionally its involvement in these pathways links it to other related proteins like Axin which help modulate signal transduction.

GSKIP has connections to cancer and neurodegenerative diseases partly due to its role in regulating GSK3β activity. Alterations in the pathways involving GSKIP can impact disease progression and response to treatment. In cancer dysregulation of the Wnt pathway through GSKIP interactions may lead to abnormal cell proliferation. In neurodegenerative diseases its interaction with GSK3β relates to the phosphorylation of tau protein a significant aspect of Alzheimer's disease pathology.
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Specifications

Form

Liquid

Additional notes

ab105621 was purified using conventional chromatography techniques.

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General info

Function

A-kinase anchoring protein for GSK3B and PKA that regulates or facilitates their kinase activity towards their targets (PubMed : 16981698, PubMed : 25920809, PubMed : 27484798). The ternary complex enhances Wnt-induced signaling by facilitating the GSK3B- and PKA-induced phosphorylation of beta-catenin leading to beta-catenin degradation and stabilization respectively (PubMed : 16981698, PubMed : 27484798). Upon cAMP activation, the ternary complex contributes to neuroprotection against oxidative stress-induced apoptosis by facilitating the PKA-induced phosphorylation of DML1 and PKA-induced inactivation of GSK3B (PubMed : 25920809). During neurite outgrowth promotes neuron proliferation; while increases beta-catenin-induced transcriptional activity through GSK3B kinase activity inhibition, reduces N-cadherin level to promote cell cycle progression (PubMed : 19830702).

Sequence similarities

Belongs to the GSKIP family.

Post-translational modifications

Phosphorylated by GSK3B.

Subcellular localisation

Nucleus

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Product protocols

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Target data

A-kinase anchoring protein for GSK3B and PKA that regulates or facilitates their kinase activity towards their targets (PubMed : 16981698, PubMed : 25920809, PubMed : 27484798). The ternary complex enhances Wnt-induced signaling by facilitating the GSK3B- and PKA-induced phosphorylation of beta-catenin leading to beta-catenin degradation and stabilization respectively (PubMed : 16981698, PubMed : 27484798). Upon cAMP activation, the ternary complex contributes to neuroprotection against oxidative stress-induced apoptosis by facilitating the PKA-induced phosphorylation of DML1 and PKA-induced inactivation of GSK3B (PubMed : 25920809). During neurite outgrowth promotes neuron proliferation; while increases beta-catenin-induced transcriptional activity through GSK3B kinase activity inhibition, reduces N-cadherin level to promote cell cycle progression (PubMed : 19830702).
See full target information GSKIP
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