Recombinant Human GST-1 protein is a Human Fragment protein, in the 168 to 267 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
P P G P A D L V L E E G D C V R K C G L L N L T V A A E A C R E R S H V A I K T V R V P E V N D L R A L V E D P R L N L K V I Q L V R D P R G I L A S R S E T F R D T Y R L W R L W Y G T G R K P Y N L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of internal galactose (Gal) residues of keratan. Cooperates with B4GALT4 and B3GNT7 glycosyltransferases and CHST6 sulfotransferase to construct and elongate disulfated disaccharide unit [->3(6-sulfoGalbeta)1->4(6-sulfoGlcNAcbeta)1->] within keratan sulfate polymer (PubMed:10642612, PubMed:17690104, PubMed:9405439). Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer (PubMed:9405439). Involved in biosynthesis of phosphacan, a major keratan sulfate proteoglycan in the developing brain (By similarity). Involved in biosynthesis of 6-sulfoGalbeta-containing O-linked glycans in high endothelial venules of lymph nodes. May act in a synergistic manner with CHST4 to generate sialyl 6',6-disulfo Lewis X motif, a recognition determinant for immune cell receptors implicated in leukocyte trafficking (PubMed:10330415). Catalyzes sulfation of N-acetyllactosamine (LacNAc) oligosaccharides with highest efficiency for sialylated LacNAc structures (PubMed:10642612).
Alternative names
Carbohydrate sulfotransferase 1, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1, Keratan sulfate Gal-6 sulfotransferase, GST-1, KS6ST, KSGal6ST, KSST, CHST1
Recommended products
Recombinant Human GST-1 protein is a Human Fragment protein, in the 168 to 267 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- O43916-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- P P G P A D L V L E E G D C V R K C G L L N L T V A A E A C R E R S H V A I K T V R V P E V N D L R A L V E D P R L N L K V I Q L V R D P R G I L A S R S E T F R D T Y R L W R L W Y G T G R K P Y N L
- Accession
- O43916
- Protein length
- Fragment
- Predicted molecular weight
- 36.74 kDa
- Amino acids
- 168 to 267
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of internal galactose (Gal) residues of keratan. Cooperates with B4GALT4 and B3GNT7 glycosyltransferases and CHST6 sulfotransferase to construct and elongate disulfated disaccharide unit [->3(6-sulfoGalbeta)1->4(6-sulfoGlcNAcbeta)1->] within keratan sulfate polymer (PubMed:10642612, PubMed:17690104, PubMed:9405439). Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer (PubMed:9405439). Involved in biosynthesis of phosphacan, a major keratan sulfate proteoglycan in the developing brain (By similarity). Involved in biosynthesis of 6-sulfoGalbeta-containing O-linked glycans in high endothelial venules of lymph nodes. May act in a synergistic manner with CHST4 to generate sialyl 6',6-disulfo Lewis X motif, a recognition determinant for immune cell receptors implicated in leukocyte trafficking (PubMed:10330415). Catalyzes sulfation of N-acetyllactosamine (LacNAc) oligosaccharides with highest efficiency for sialylated LacNAc structures (PubMed:10642612).
- Sequence similarities
Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Carbohydrate sulfotransferase 1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GST-1 also known as Glutathione S-transferase 1 is an enzyme with a molecular mass of around 26 kDa. This target works mechanically by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. GST-1 shows expression in various tissues including the liver which is a major site for drug metabolism. It also plays a role in antioxidant defense helping to protect cells from damage by reactive oxygen species (ROS).
- Biological function summary
Within cellular contexts GST-1 performs critical functions related to the body's ability to handle oxidative stress. This enzyme does not typically operate as part of a larger complex but works individually or in collaboration with other GST isoenzymes. It contributes to the detoxification processes by neutralizing harmful compounds and maintaining cellular health. GST-1 also impacts the bioavailability and breakdown of hormones drugs and other xenobiotics.
- Pathways
GST-1 plays significant roles within oxidative stress response and detoxification pathways. It participates in the pathway that involves glutathione metabolism protecting cells from oxidative damage. GST-1 acts in conjunction with other antioxidant proteins like superoxide dismutase (SOD) and catalase working together to effectively minimize ROS levels. This cooperation helps maintain cellular homeostasis and prevents damage that can lead to various pathological conditions.
- Associated diseases and disorders
GST-1 shows associations with cancer and neurodegenerative disorders. Alterations in GST-1 expression levels can influence the susceptibility to certain cancers including liver and lung cancers where detoxification capacity becomes compromised. In neurodegenerative disorders like Parkinson's disease GST-1 plays a protective role where its interaction with other GST isoenzymes could potentially affect neuronal survival. Understanding these connections helps in exploring therapeutic interventions for managing oxidative stress-related diseases.
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1 product image
SDS-PAGE - Recombinant Human GST-1 protein (ab152884)
12.5% SDS-PAGE analysis of ab152884 stained with Coomassie Blue.
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