JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB268549

Recombinant human GST-FAM131b-BRAF fusion protein (Active) (GST tag N-Terminus, Tag Free)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human GST-FAM131b-BRAF fusion protein (Active) (GST tag N-Terminus, Tag Free) is a Human Fragment protein, in the 1 to 30 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.
2 Images
Functional Studies - Recombinant human GST-FAM131b-BRAF fusion protein (Active) (GST tag N-Terminus, Tag Free) (AB268549)
  • FuncS

Supplier Data

Functional Studies - Recombinant human GST-FAM131b-BRAF fusion protein (Active) (GST tag N-Terminus, Tag Free) (AB268549)

The specific activity of ab268549 was 2,100 nmol/min/mg in a coupled assay using MBP as substrate.

SDS-PAGE - Recombinant human GST-FAM131b-BRAF fusion protein (Active) (GST tag N-Terminus, Tag Free) (AB268549)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human GST-FAM131b-BRAF fusion protein (Active) (GST tag N-Terminus, Tag Free) (AB268549)

SDS-PAGE analysis of ab268549.

Key facts

Purity

>70% SDS-PAGE

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab268549 was 2,100 nmol/min/mg in a coupled assay using MBP as substrate.

Accession

P15056-1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB228461

Anti-BRAF (mutated V600E) antibody [VE1]

20ul selling size|BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF (mutated V600E) antibody [VE1] (AB228461)

  • 4
  • 7
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB313357

Recombinant Human NRAS Protein (His tag) (E.Coli)

Mass Spectrometry - Recombinant Human NRAS Protein (His tag) (E.Coli) (AB313357)

  • 0
  • 0
Proteins & Peptides

AB60755

Recombinant mouse PDGFR alpha protein (GST tag N-Terminus)

Functional Studies - Recombinant mouse PDGFR alpha protein (GST tag N-Terminus) (AB60755)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

GST-FAM131B-BRAF fusion protein.
style
left-column

Sequence info

[{"linker":null,"sequence":"MDSTSSLHGSSLHRPSTEQTRTDFSWDGIN","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":30,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q86XD5","tags":[{"tag":"GST","terminus":"N-Terminus"}]},{"linker":null,"sequence":"DLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":766,"aminoAcidStart":381,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P15056","tags":[]}]
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column
style
left-column

General info

Function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

Post-translational modifications

Phosphorylation at Ser-365 by SGK1 inhibits its activity (Ref.8, PubMed:11410590). Phosphorylation at Thr-753 by MAPK1 (PubMed:19710016). Dephosphorylation of Ser-365 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC); this relieves inactivation and stimulates kinase activity (PubMed:35768504, PubMed:35830882).. Methylation at Arg-671 decreases stability and kinase activity.. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).
See full target information BRAF

Additional targets

FAM131B

Alternative Names

BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, KIAA0773, FAM131B, Protein FAM131B
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com