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AB268708

Recombinant human GST-KIF5B Ret (V804L) fusion protein (Active)

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Recombinant human GST-KIF5B Ret (V804L) fusion protein (Active) is a Human Fragment protein, in the 1 to 575 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KNS, KNS1, KIF5B, Kinesin-1 heavy chain, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, UKHC

2 Images
Functional Studies - Recombinant human GST-KIF5B Ret (V804L) fusion protein (Active) (AB268708)
  • FuncS

Supplier Data

Functional Studies - Recombinant human GST-KIF5B Ret (V804L) fusion protein (Active) (AB268708)

The specific activity of ab268708 was determined to be 49 nmol/min/mg in a kinase assay using IGF1Rtide (KKKSPGEYVNIEFG) synthetic peptide substrate.

SDS-PAGE - Recombinant human GST-KIF5B Ret (V804L) fusion protein (Active) (AB268708)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human GST-KIF5B Ret (V804L) fusion protein (Active) (AB268708)

SDS-PAGE analysis of ab268708.

Key facts

Purity

>75% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab268708 was determined to be 49 nmol/min/mg in a kinase assay using IGF1Rtide (KKKSPGEYVNIEFG) synthetic peptide substrate.

Accession

P33176

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

GST-KIF5B-Ret (V804L) fusion protein.
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Sequence info

[{"sequence":"MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVK","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":575,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P33176","tags":[{"tag":"GST","terminus":"N-Terminus"}]},{"sequence":"713-end","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P07949","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target known as KIF5B-RET is a fusion protein that results from the rearrangement between the KIF5B (kinesin family member 5B) gene and the RET (rearranged during transfection) proto-oncogene. This fusion protein retains the motor domain of KIF5B and the tyrosine kinase domain of RET. KIF5B has a molecular mass of approximately 116 kDa and RET is about 170 kDa when forming its normal protein product. KIF5B is largely expressed in neuronal tissues while RET is commonly found in tissues of the embryonic kidney neural crest derivatives and other neuroepithelial-derived tissues.
Biological function summary

The formation of the KIF5B-RET fusion results in a constitutively active tyrosine kinase leading to unregulated cell signaling. KIF5B-RET is not known to be part of a larger stable protein complex but it initiates signaling pathways essential for cell proliferation and differentiation. The fusion protein hijacks the motor function of KIF5B to position RET inappropriately within the cell which leads to constitutive activation of downstream signaling cascades.

Pathways

The constitutive activity of the KIF5B-RET fusion protein impacts the MAPK (mitogen-activated protein kinase) and PI3K-AKT (phosphoinositide 3-kinase/protein kinase B) signaling pathways. These pathways are critical for regulating cell growth and survival and the KIF5B-RET fusion protein abnormally stimulates them. The natural RET protein associates with ligands such as GDNF (glial cell-derived neurotrophic factor) but the fusion bypasses the need for these ligands resulting in active signaling independent of external cues.

KIF5B-RET is prominently associated with certain types of lung cancer specifically non-small cell lung cancer (NSCLC) and thyroid cancer particularly papillary thyroid carcinoma. The presence of this fusion leads to uncontrolled proliferation of cancerous cells. RET is also connected to other proteins such as ALK (anaplastic lymphoma kinase) in these cancers as they both drive oncogenic pathways leading to tumor development and progression. Identifying KIF5B-RET fusions in tumors has led to targeted therapies aimed at inhibiting its kinase activity.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed : 20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity). Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends (Probable). Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (PubMed : 24088571).

Sequence similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed : 20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity). Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends (Probable). Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (PubMed : 24088571).
See full target information KIF5B mutated V804L

Additional targets

KIF5B
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