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AB268984

Recombinant human GST-Striatin-ALK fusion protein (Active)

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Recombinant human GST-Striatin-ALK fusion protein (Active) is a Human Fragment protein, in the 1 to 137 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Striatin, STRN

2 Images
Functional Studies - Recombinant human GST-Striatin-ALK fusion protein (Active) (AB268984)
  • FuncS

Supplier Data

Functional Studies - Recombinant human GST-Striatin-ALK fusion protein (Active) (AB268984)

The specific activity of ab268984 was 19.5 nmol/min/mg in a peptide kinase assay using IGF1Rtide as substrate.

SDS-PAGE - Recombinant human GST-Striatin-ALK fusion protein (Active) (AB268984)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human GST-Striatin-ALK fusion protein (Active) (AB268984)

SDS-PAGE analysis of ab268984.

Key facts

Purity

>85% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab268984 was 19.5 nmol/min/mg in a peptide kinase assay using IGF1Rtide as substrate.

Accession

O43815

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

GST-Striatin-ALK fusion protein.
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Sequence info

[{"sequence":"MDEQAGPGVFFSNNHPGAGGAKGLGPLAEAAAAGDGAAAAGAARAQYSLPGILHFLQHEWARFEVERAQWEVERAELQAQIAFLQGERKGQENLKKDLVRRIKMLEYALKQERAKYHKLKYGTELNQGDMKPPSYDS","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":137,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O43815","tags":[{"tag":"GST","terminus":"N-Terminus"}]},{"sequence":"VYRRKHQELQAMQMELQSPEYKLSKLRTSTIMTDYNPNYCFAGKTSSISDLKEVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALIISKFNHQNIVRCIGVSLQSLPRFILLELMAGGDLKSFLRETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPGPGRVAKIGDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPVYRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEEKVPVRPKDPEGVPPLLVSQQAKREEERSPAAPPPLPTTSSGKAAKKPTAAEISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGSWFTEKPTKKNNPIAKKEPHDRGNLGLEGSCTVPPNVATGRLPGASLLLEPSSLTANMKEVPLFRLRHFPCGNVNYGYQQQGLPLEAATAPGAGHYEDTILKSKNSMNQPGP","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1620,"aminoAcidStart":1058,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UM73","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ALK (Anaplastic Lymphoma Kinase) also known as CD246 is a receptor tyrosine kinase with a molecular weight of approximately 176 kDa. It is mainly expressed in the central nervous system particularly within the brain but also found in peripheral tissues. The striatin a protein interacts with ALK and acts as a scaffolding protein. It plays a role in organizing other proteins into larger complexes. The expression of ALK is more abundant in certain cell types such as neurons but its presence is also noted in some cancer cells where it contributes to abnormal cell proliferation.
Biological function summary

ALK functions as a critical regulator of cell growth and survival often involved in the signaling pathways of the nervous system. It forms complexes with several intracellular proteins indicating its role in intricate signaling networks. Upon binding of ligands ALK undergoes autophosphorylation which triggers downstream signaling cascades that influence various cellular processes including neuronal development and synapse formation. Striatin in its role as a scaffolding protein helps organize these signaling proteins making the pathway more efficient.

Pathways

ALK links closely to the PI3K/AKT and RAS/ERK signaling pathways which are important for cell proliferation and survival. These pathways are often altered in cancers making ALK a target of interest in oncology research. Through the PI3K/AKT pathway ALK interacts with proteins like PDK1 and mTOR influencing cell cycle progression and apoptosis. In the RAS/ERK pathway ALK contributes to cellular responses to external growth signals. The interaction of ALK with these pathways highlights its role in maintaining proper cellular communication and function.

ALK alterations associate with several cancers including neuroblastoma and non-small cell lung cancer. Its fusion or mutation leads to continuous activation driving oncogenic processes. In non-small cell lung cancer ALK often partners with the EML4 protein through gene rearrangement resulting in the EML4-ALK fusion protein that exhibits constant activity promoting tumor growth. Similarly in neuroblastoma ALK mutations result in sustained signalling and proliferation. Understanding these connections allows for targeted therapy development seeking to inhibit the aberrant activity of ALK in these malignancies.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Calmodulin-binding scaffolding protein which is the center of the striatin-interacting phosphatase and kinase (STRIPAK) complexes (PubMed : 18782753). STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (Probable).

Sequence similarities

Belongs to the WD repeat striatin family.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calmodulin-binding scaffolding protein which is the center of the striatin-interacting phosphatase and kinase (STRIPAK) complexes (PubMed : 18782753). STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (Probable).
See full target information Striatin

Additional targets

ALK
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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