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AB86703

Recombinant human GST3 / GST pi protein (His tag N-Terminus)

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Recombinant human GST3 / GST pi protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

FAEES3, GST3, GSTP1, Glutathione S-transferase P, GST class-pi, GSTP1-1

2 Images
Western blot - Recombinant human GST3 / GST pi protein (His tag N-Terminus) (AB86703)
  • WB

Unknown

Western blot - Recombinant human GST3 / GST pi protein (His tag N-Terminus) (AB86703)

All lanes:

Anti-GST3 / GST pi antibody - Carboxyterminal end (<a href='/en-us/products/unavailable/gst3-gst-pi-antibody-carboxyterminal-end-ab65977'>ab65977</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant human GST3 / GST pi protein (His tag N-Terminus) (ab86703) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 12min

SDS-PAGE - Recombinant human GST3 / GST pi protein (His tag N-Terminus) (AB86703)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human GST3 / GST pi protein (His tag N-Terminus) (AB86703)

15% SDS-PAGE showing ab86703 at approximately 27.6kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 80 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Accession

P09211

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Constituents: 30% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.03% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab86703 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/gst3-gst-pi-antibody-carboxyterminal-end-ab65977'>ab65977</a>.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSHMPPYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPKFQDGDLTLYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYISLIYTNYEAGKDDYVKALPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVGRLSARPKLKAFLASPEYVNLPINGNGKQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P09211","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST3 also known as GST pi or GSTP is a protein part of the glutathione S-transferase family. These enzymes are involved in detoxifying processes by catalyzing the conjugation of glutathione to harmful substances. GSTP is approximately 23 kDa in size and is predominantly expressed in mammalian tissues including the liver and lung. These tissues play active roles in detoxifying external chemicals and managing oxidative stress in the body.
Biological function summary

GST3 contributes to the detoxification of xenobiotics and electrophilic compounds playing a protective role against cellular damage. It can exist as homo-dimers or form hetero-dimers with other GST family members participating in larger enzyme complexes. Through conjugation reactions GSTP helps in the detoxification processes thereby aiding cellular defenses and maintaining cellular redox balance.

Pathways

GST3 is an integral part of the phase II detoxification pathway and oxidative stress response. It interacts closely with other proteins such as Nrf2 (Nuclear factor erythroid 2-related factor 2) and helps regulate the transcription of antioxidant proteins that protect against oxidative damage. This enzyme plays a role in modulating the MAPK signaling pathway which influences cell proliferation and apoptosis.

GSTP has links to several cancer types including liver and lung cancer. Its overexpression in tumors suggests its role in cancer cell survival and resistance to chemotherapy. GSTP interacts with cancer-associated proteins like c-Jun N-terminal kinase (JNK) influencing pathways that lead to aberrant cellular growth and transformation. It also has associations with neurodegenerative diseases given its role in counteracting oxidative stress which commonly underpins neurodegeneration.
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Specifications

Form

Liquid

Additional notes

ab86703 is purified using conventional chromatography techniques.Endotoxin level: < 1.0 EU per 1 μg of protein (determined by LAL method).

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General info

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.

Sequence similarities

Belongs to the GST superfamily. Pi family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
See full target information GSTP1
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Complementary Products

Primary Antibodies

AB138491

Anti-GST3 / GST pi antibody [EPR8263]

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Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] (AB138491)

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Primary Antibodies

AB183340

Anti-Nucleophosmin antibody [SP236]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] (AB183340)

  • 0
  • 0

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