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AB206797

Recombinant human GSTA4 protein (His tag N-Terminus)

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Recombinant human GSTA4 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 222 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, Mass Spec.

View Alternative Names

Glutathione S-transferase A4, GST class-alpha member 4, Glutathione S-transferase A4-4, GSTA4

1 Images
SDS-PAGE - Recombinant human GSTA4 protein (His tag N-Terminus) (AB206797)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human GSTA4 protein (His tag N-Terminus) (AB206797)

15% SDS-PAGE analysis of ab206797 (3 μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, Mass Spec, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 degC.

Accession

O15217

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Proteins & Peptides

AB276403

Recombinant Human VSIG2 protein (His tag)

SDS-PAGE - Recombinant Human VSIG2 protein (His tag) (AB276403)

  • 0
  • 0
Proteins & Peptides

AB87361

Recombinant Human 14-3-3 zeta protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human 14-3-3 zeta protein (AB87361)

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Primary Antibodies

AB248729

Anti-GSTA4 antibody [EPR8404(B)] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-GSTA4 antibody [EPR8404(B)] - BSA and Azide free (AB248729)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMAARPKLHYPNGRGRMESVRWVLAAAGVEFDEEFLETKEQLYKLQDGNHLLFQQVPMVEIDGMKLVQTRSILHYIADKHNLFGKNLKERTLIDMYVEGTLDLLELLIMHPFLKPDDQQKEVVNMAQKAIIRYFPVFEKILRGHGQSFLVGNQLSLADVILLQTILALEEKIPNILSAFPFLQEYTVKLSNIPTIKRFLEPGSKKKPPPDEIYVRTVYNIFRP","proteinLength":"Full Length","predictedMolecularWeight":"28.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":222,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O15217","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GSTA4 also known as Glutathione S-transferase alpha-4 is an enzyme that plays a critical role in detoxification processes. This protein weighs approximately 25 kDa and exhibits strong expression in tissues such as the liver kidney and brain. GSTA4 belongs to the glutathione S-transferase superfamily specifically the alpha class which is involved in the conjugation of the reduced form of glutathione (GSH) to a wide range of hydrophobic electrophiles.
Biological function summary

GSTA4 helps in the metabolism of lipid peroxidation products by catalyzing their conjugation with glutathione. This process is essential for reducing oxidative stress caused by cellular metabolism. GSTA4 does not normally function as part of a larger complex but instead works independently to carry out this detoxification role. The ability of GSTA4 to target lipid peroxidation products is significant for cellular protection mechanisms.

Pathways

GSTA4 actively participates in the detoxification pathway where it neutralizes harmful compounds like 4-hydroxynonenal (4-HNE) a byproduct of lipid peroxidation. GSTA4 is also involved in the glutathione metabolism pathway playing an important role alongside other GST isoforms in managing oxidative stress within cells. Proteins related through these pathways include other members of the glutathione S-transferase family which share similar functional roles in detoxification processes.

GSTA4 impacts the development of conditions related to oxidative stress such as Alzheimer's disease and cancer. Through its detoxifying activity GSTA4 helps mitigate oxidative damage that may contribute to the progression of these diseases. Research indicates the dysregulation of GSTA4 expression links to both Alzheimer's disease and cancer along with its interactions with other GST family proteins highlighting its importance in disease pathology.
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Specifications

Form

Liquid

Additional notes

ab206797 was expressed in E.coli and purified by using conventional chromatography techniques.

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General info

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).

Sequence similarities

Belongs to the GST superfamily. Alpha family.

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Product protocols

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).
See full target information Glutathione S-transferase A4
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Product promise

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