Recombinant Human GSTM3 protein is a Human Full Length protein, in the 1 to 225 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M S C E S S M V L G Y W D I R G L A H A I R L L L E F T D T S Y E E K R Y T C G E A P D Y D R S Q W L D V K F K L D L D F P N L P Y L L D G K N K I T Q S N A I L R Y I A R K H N M C G E T E E E K I R V D I I E N Q V M D F R T Q L I R L C Y S S D H E K L K P Q Y L E E L P G Q L K Q F S M F L G K F S W F A G E K L T F V D F L T Y D I L D Q N R I F D P K C L D E F P N L K A F M C R F E A L E K I A A Y L Q S D Q F C K M P I N N K M A Q W G N K P V C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.
Alternative names
GST5, GSTM3, Glutathione S-transferase Mu 3, GST class-mu 3, GSTM3-3, hGSTM3-3
Recommended products
Recombinant Human GSTM3 protein is a Human Full Length protein, in the 1 to 225 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P21266-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M S C E S S M V L G Y W D I R G L A H A I R L L L E F T D T S Y E E K R Y T C G E A P D Y D R S Q W L D V K F K L D L D F P N L P Y L L D G K N K I T Q S N A I L R Y I A R K H N M C G E T E E E K I R V D I I E N Q V M D F R T Q L I R L C Y S S D H E K L K P Q Y L E E L P G Q L K Q F S M F L G K F S W F A G E K L T F V D F L T Y D I L D Q N R I F D P K C L D E F P N L K A F M C R F E A L E K I A A Y L Q S D Q F C K M P I N N K M A Q W G N K P V C
- Accession
- P21266
- Protein length
- Full Length
- Predicted molecular weight
- 29.1 kDa
- Amino acids
- 1 to 225
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab124587 is purified using conventional chromatography techniques.
General info
- Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.
- Sequence similarities
Belongs to the GST superfamily. Mu family.
- Post-translational modifications
The N-terminus is blocked.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GSTM3 also known as Glutathione S-transferase Mu 3 is a protein that plays a role in detoxification processes. This enzyme belongs to the GST superfamily which assists in conjugating glutathione to hydrophobic and electrophilic compounds. GSTM3 has a molecular weight of approximately 26 kDa. It is expressed in various tissues including the brain liver and testis indicating its involvement in diverse physiological functions.
- Biological function summary
GSTM3 facilitates the neutralization of reactive oxygen species protecting cells from oxidative damage. This protein interacts with intracellular substrates engaging in phase II metabolic reactions. While GSTM3 does not form larger multimeric complexes it partners with glutathione to stabilize and detoxify harmful compounds. This role highlights GSTM3’s importance within cellular defense mechanisms.
- Pathways
GSTM3 participates in the xenobiotic metabolism and the glutathione pathway. These pathways involve the biotransformation of toxic substances where GSTM3 works in tandem with related proteins like CYP450 enzymes. GSTM3's activity in the glutathione pathway interconnects with the regulation of oxidative stress highlighting its part in cellular resilience against environmental challenges.
- Associated diseases and disorders
GSTM3 has connections to neurological disorders and certain cancers. Its involvement in detoxification processes links it to the neurodegenerative condition Parkinson's disease where oxidative stress plays a critical role. In certain cancers variations in GSTM3 expression levels influence tumor development and aggressiveness. GSTM3's interaction with proteins involved in these conditions such as GSTP1 offers potential insights into therapeutic strategies.
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1 product image
SDS-PAGE - Recombinant Human GSTM3 protein (ab124587)
15% SDS-PAGE showing ab124587 at approximately 29.1kDa (3μg).
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