Recombinant Human GSTZ1 protein is a Human Full Length protein, in the 1 to 216 aa range, expressed in Escherichia coli, with >95% purity, = 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- = 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, HPLC
- Biologically active
- No
Amino acid sequence
M Q A G K P I L Y S Y F R S S C S W R V R I A L A L K G I D Y E T V P I N L I K D G G Q Q F S K D F Q A L N P M K Q V P T L K I D G I T I H Q S L A I I E Y L E E M R P T P R L L P Q D P K K R A S V R M I S D L I A G G I Q P L Q N L S V L K Q V G E E M Q L T W A Q N A I T C G F N A L E Q I L Q S T A G I Y C V G D E V T M A D L C L V P Q V A N A E R F K V D L T P Y P T I S S I N K R L L V L E A F Q V S H P C R Q P D T P T E L R A L E H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid.
Alternative names
MAAI, GSTZ1, Maleylacetoacetate isomerase, GSTZ1-1, Glutathione S-transferase zeta 1
Recommended products
Recombinant Human GSTZ1 protein is a Human Full Length protein, in the 1 to 216 aa range, expressed in Escherichia coli, with >95% purity, = 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- = 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, HPLC
- Biologically active
- No
- Accession
- O43708-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M Q A G K P I L Y S Y F R S S C S W R V R I A L A L K G I D Y E T V P I N L I K D G G Q Q F S K D F Q A L N P M K Q V P T L K I D G I T I H Q S L A I I E Y L E E M R P T P R L L P Q D P K K R A S V R M I S D L I A G G I Q P L Q N L S V L K Q V G E E M Q L T W A Q N A I T C G F N A L E Q I L Q S T A G I Y C V G D E V T M A D L C L V P Q V A N A E R F K V D L T P Y P T I S S I N K R L L V L E A F Q V S H P C R Q P D T P T E L R A L E H H H H H H
- Accession
- O43708
- Protein length
- Full Length
- Predicted molecular weight
- 25 kDa
- Amino acids
- 1 to 216
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
General info
- Function
Bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid.
- Sequence similarities
Belongs to the GST superfamily. Zeta family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Glutathione Transferase zeta 1
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GSTZ1 also known as Glutathione S-Transferase Zeta 1 is an enzyme that plays an important role in the metabolism of various chemical compounds. It has a molecular mass of approximately 34 kDa. GSTZ1 is ubiquitously expressed in many tissues with significant levels in the liver kidney and heart. This enzyme is part of the GST family which contributes to detoxification processes by conjugating reduced glutathione to various substrates.
- Biological function summary
GSTZ1 participates in the breakdown of amino acids specifically in the catabolic pathway of L-phenylalanine and L-tyrosine. The enzyme assists in the production of maleylacetoacetate and its isomerization to fumarylacetoacetate an essential step in the phenylalanine and tyrosine catabolism. GSTZ1 does not form part of a larger multi-protein complex but its function is critical in maintaining cellular homeostasis by preventing the accumulation of potentially toxic intermediates.
- Pathways
GSTZ1 integrates into the metabolic pathway that includes glutathione metabolism and the phenylalanine and tyrosine degradation pathway. It interacts functionally with other enzymes like Fumarylacetoacetate hydrolase (FAH) and Phenylalanine hydroxylase (PAH) playing roles in sequential catalysis steps. These pathways are vital for maintaining amino acid balance and detoxification of byproducts within the organism.
- Associated diseases and disorders
GSTZ1 has been linked to metabolic disorders like Tyrosinemia type I and various forms of cancer. Tyrosinemia type I a disorder caused by defects in tyrosine metabolism directly implicates GSTZ1 as an important player in managing toxic intermediates. Additionally altered expression of GSTZ1 has been observed in different cancer types suggesting its potential role in tumorigenesis. FAH related through the Tyrosinemia disorder represents another key protein that GSTZ1 activity impacts highlighting its disease association.
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1 product image
SDS-PAGE - Recombinant Human GSTZ1 protein (ab172170)
SDS-PAGE gel of ab172170 under non reduced (lane 1) and reduced (lane 2) conditions.
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