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AB81890

Recombinant Human GTF2H1 protein

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Recombinant Human GTF2H1 protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

BTF2, GTF2H1, General transcription factor IIH subunit 1, Basic transcription factor 2 62 kDa subunit, General transcription factor IIH polypeptide 1, TFIIH basal transcription factor complex p62 subunit, BTF2 p62

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P32780

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.9 Constituents: 20% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P32780","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GTF2H1 also known as Transcription Factor IIH Subunit 1 is a protein involved in transcription and DNA repair processes. The protein weighs approximately 71 kDa and consists of 667 amino acids. It functions as a part of the TFIIH complex. GTF2H1 is expressed in various tissues with higher levels observed in actively dividing cells suggesting its significant role in cellular proliferation activities. The protein’s complex interactions are indispensable for several DNA-dependent processes.
Biological function summary

GTF2H1 is a component of the TFIIH complex which has roles in both transcription initiation and nucleotide excision repair (NER). This complex is vital for the accurate transcription of DNA into RNA recognizing transcription factors and facilitating chromatin remodeling. Within NER GTF2H1 participates in unwinding DNA allowing the repair machinery to access damaged regions. Its activity ensures the maintenance of genomic stability which is essential for preventing mutations.

Pathways

GTF2H1 plays significant roles in the transcription initiation pathway and the DNA repair pathway. The protein interacts intricately with other members of the TFIIH complex like XPD and XPB which are helicases that modulate DNA unwinding during transcription and repair. These interactions are essential for coordinating the cellular response to DNA damage and regulating transcription by RNA polymerase II integrating the response with cell cycle control mechanisms.

Mutations or dysfunctions of GTF2H1 can lead to conditions like xeroderma pigmentosum and trichothiodystrophy. Both disorders are associated with defects in the nucleotide excision repair process. In these conditions proteins like XPD (also a part of TFIIH) are implicated which highlights the critical nature of GTF2H1 in maintaining DNA integrity. Such associations highlight the importance of thorough functional understanding of GTF2H1 in the development of therapeutic interventions.
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Specifications

Form

Liquid

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General info

Function

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.

Sequence similarities

Belongs to the TFB1 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
See full target information GTF2H1
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