Recombinant human GWL protein (Active) is a Human Full Length protein, in the 1 to 879 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M D P T A G S K K E P G G G A A T E E G V N R I A V P K P P S I E E F S I V K P I S R G A F G K V Y L G Q K G G K L Y A V K V V K K A D M I N K N M T H Q V Q A E R D A L A L S K S P F I V H L Y Y S L Q S A N N V Y L V M E Y L I G G D V K S L L H I Y G Y F D E E M A V K Y I S E V A L A L D Y L H R H G I I H R D L K P D N M L I S N E G H I K L T D F G L S K V T L N R D I N M M D I L T T P S M A K P R Q D Y S R T P G Q V L S L I S S L G F N T P I A E K N Q D P A N I L S A C L S E T S Q L S Q G L V C P M S V D Q K D T T P Y S S K L L K S C L E T V A S N P G M P V K C L T S N L L Q S R K R L A T S S A S S Q S H T F I S S V E S E C H S S P K W E K D C Q E S D E A L G P T M M S W N A V E K L C A K S A N A I E T K G F N K K D L E L A L S P I H N S S A L P T T G R S C V N L A K K C F S G E V S W E A V E L D V N N I N M D T D T S Q L G F H Q S N Q W A V D S G G I S E E H L G K R S L K R N F E L V D S S P C K K I I Q N K K T C V E Y K H N E M T N C Y T N Q N T G L T V E V Q D L K L S V H K S Q Q N D C A N K E N I V N S F T D K Q Q T P E K L P I P M I A K N L M C E L D E D C E K N S K R D Y L S S S F L C S D D D R A S K N I S M N S D S S F P G I S I M E S P L E S Q P L D S D R S I K E S S F E E S N I E D P L I V T P D C Q E K T S P K G V E N P A V Q E S N Q K M L G P P L E V L K T L A S K R N A V A F R S F N S H I N A S N N S E P S R M N M T S L D A M D I S C A Y S G S Y P M A I T P T Q K R R S C M P H Q Q T P N Q I K S G T P Y R T P K S V R R G V A P V D D G R I L G T P D Y L A P E L L L G R A H G P A V D W W A L G V C L F E F L T G I P P F N D E T P Q Q V F Q N I L K R D I P W P E G E E K L S D N A Q S A V E I L L T I D D T K R A G M K E L K R H P L F S D V D W E N L Q H Q T M P F I P Q P D D E T D T S Y F E A R N T A Q H L T V S G F S L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
Alternative names
GW, GWL, THC2, MASTL, Serine/threonine-protein kinase greatwall, hGWL, Microtubule-associated serine/threonine-protein kinase-like, MAST-L
Recommended products
Recombinant human GWL protein (Active) is a Human Full Length protein, in the 1 to 879 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity was determined to be 16.2 nmol/min/mg in a kinase assay using ARPP19 protein substrate.
- Accession
- Q96GX5-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M D P T A G S K K E P G G G A A T E E G V N R I A V P K P P S I E E F S I V K P I S R G A F G K V Y L G Q K G G K L Y A V K V V K K A D M I N K N M T H Q V Q A E R D A L A L S K S P F I V H L Y Y S L Q S A N N V Y L V M E Y L I G G D V K S L L H I Y G Y F D E E M A V K Y I S E V A L A L D Y L H R H G I I H R D L K P D N M L I S N E G H I K L T D F G L S K V T L N R D I N M M D I L T T P S M A K P R Q D Y S R T P G Q V L S L I S S L G F N T P I A E K N Q D P A N I L S A C L S E T S Q L S Q G L V C P M S V D Q K D T T P Y S S K L L K S C L E T V A S N P G M P V K C L T S N L L Q S R K R L A T S S A S S Q S H T F I S S V E S E C H S S P K W E K D C Q E S D E A L G P T M M S W N A V E K L C A K S A N A I E T K G F N K K D L E L A L S P I H N S S A L P T T G R S C V N L A K K C F S G E V S W E A V E L D V N N I N M D T D T S Q L G F H Q S N Q W A V D S G G I S E E H L G K R S L K R N F E L V D S S P C K K I I Q N K K T C V E Y K H N E M T N C Y T N Q N T G L T V E V Q D L K L S V H K S Q Q N D C A N K E N I V N S F T D K Q Q T P E K L P I P M I A K N L M C E L D E D C E K N S K R D Y L S S S F L C S D D D R A S K N I S M N S D S S F P G I S I M E S P L E S Q P L D S D R S I K E S S F E E S N I E D P L I V T P D C Q E K T S P K G V E N P A V Q E S N Q K M L G P P L E V L K T L A S K R N A V A F R S F N S H I N A S N N S E P S R M N M T S L D A M D I S C A Y S G S Y P M A I T P T Q K R R S C M P H Q Q T P N Q I K S G T P Y R T P K S V R R G V A P V D D G R I L G T P D Y L A P E L L L G R A H G P A V D W W A L G V C L F E F L T G I P P F N D E T P Q Q V F Q N I L K R D I P W P E G E E K L S D N A Q S A V E I L L T I D D T K R A G M K E L K R H P L F S D V D W E N L Q H Q T M P F I P Q P D D E T D T S Y F E A R N T A Q H L T V S G F S L
- Accession
- Q96GX5
- Protein length
- Full Length
- Amino acids
- 1 to 879
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
- Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
- Post-translational modifications
Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity). Maximum phosphorylation occurs in prometaphase.
- Subcellular localisation
- Cytoskeleton, Microtubule organizing center, Centrosome, Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GWL also known as Greatwall kinase (and occasionally Mastl) is a protein kinase with a calculated molecular mass of about 58 kDa. This protein mainly expresses in various tissues but it shows higher expression in organs such as the heart liver and skeletal muscle. GWL plays a mechanical role in the regulation of cell cycle through its kinase activity which phosphorylates other key proteins involved in mitosis.
- Biological function summary
GWL influences the cell cycle and mitotic progression by modulating the activity of the phosphatase inhibitor proteins ENSA and ARPP19. It is part of a functional complex with these proteins when activated leading to the inhibition of Protein Phosphatase 2A (PP2A) during mitosis which allows for proper chromosome condensation and segregation. This ensures that the cells divide accurately maintaining genomic stability.
- Pathways
Different cellular pathways integrate their signals through GWL to regulate mitosis. One of the key pathways is the cell cycle control pathway where GWL works alongside CDK1 and is essential for the phosphorylation cascade that leads to mitotic entry. Another pathway of note is the mitotic spindle assembly checkpoint where GWL interacts with proteins such as BUB1 and MAD2 helping establish mechanisms that ensure cells do not prematurely progress through mitosis with errors.
- Associated diseases and disorders
GWL dysfunction links to cancer due to its critical role in cell division. Aberrant expression or mutation of GWL can lead to aneuploidy and genomic instability factors that contribute to tumorigenesis. Moreover abnormalities in GWL expression have been associated with neurological disorders like Alzheimer's disease where it may interact with tau protein aggregation pathways. In cancer GWL often works in conjunction with oncogenes like MYC and tumor suppressors such as p53 influencing their roles in controlling cell proliferation and survival.
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2 product images
Functional Studies - Recombinant human GWL protein (Active) (ab268748)
The specific activity was determined to be 16.2 nmol/min/mg in a kinase assay using ARPP19 protein substrate.
SDS-PAGE - Recombinant human GWL protein (Active) (ab268748)
SDS-PAGE analysis of ab268748.
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