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AB126667

Recombinant Human HAAO protein (His tag N-Terminus)

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Recombinant Human HAAO protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 286 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilic acid dioxygenase, 3-HAO, h3HAO, HAD, HAAO

1 Images
SDS-PAGE - Recombinant Human HAAO protein (His tag N-Terminus) (AB126667)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HAAO protein (His tag N-Terminus) (AB126667)

15% SDS-PAGE analysis of 3 µg ab126667.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P46952

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMERRLGVRAWVKENRGSFQPPVCNKLMHQEQLKVMFIGGPNTRKDYHIEEGEEVFYQLEGDMVLRVLEQGKHRDVVIRQGEIFLLPARVPHSPQRFANTVGLVVERRRLETELDGLRYYVGDTMDVLFEKWFYCKDLGTQLAPIIQEFFSSEQYRTGKPIPDQLLKEPPFPLSTRSIMEPMSLDAWLDSHHRELQAGTPLSLFGDTYETQVIAYGQGSSEGLRQNVDVWLWQLEGSSVVTMGGRRLSLAPDDSLLVLAGTSYAWERTQGSVALSVTQDPACKKPLG","proteinLength":"Full Length","predictedMolecularWeight":"35 kDa","actualMolecularWeight":null,"aminoAcidEnd":286,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P46952","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 3-Hydroxyanthranilate 34-dioxygenase commonly known as HAAO is an enzyme with a molecular mass of around 33 kDa. This enzyme catalyzes the conversion of 3-hydroxyanthranilate to quinolinic acid an important step in the kynurenine pathway of tryptophan metabolism. HAAO expresses strongly in the liver and kidneys with moderate expression in the brain. It is also detected in various other tissues to a lesser extent reflecting its versatile role in different physiological contexts.
Biological function summary

This enzyme plays critical roles in tryptophan catabolism linking amino acid metabolism to several critical biological processes. HAAO's activity produces quinolinic acid which is an intermediate that can further convert into NAD+. Although not part of a larger complex HAAO's function in producing quinolinic acid is important for cellular energy metabolism as NAD+ is a cofactor in redox reactions essential for maintaining energy balance in cells.

Pathways

HAAO operates predominantly within the kynurenine pathway an important route of tryptophan degradation. It connects with enzymes like kynureninase and kynurenine 3-monooxygenase which provide substrates or interact within the same pathway. This pathway not only regulates NAD+ biosynthesis but can influence neuroactive metabolites production implicating HAAO in both metabolic and neurological processes.

Alterations in HAAO activity link to certain neurodegenerative diseases including Alzheimer's disease and Huntington's disease. Elevated quinolinic acid levels a product of HAAO activity can lead to neurotoxicity and have been implicated in these conditions. Additionally studies hint at potential associations with other proteins such as IDO1 and TDO2 which could influence or modulate tryptophan metabolism and therefore affect disease processes.
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Specifications

Form

Liquid

Additional notes

ab126667 is purified using conventional chromatography techniques.

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General info

Function

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.

Sequence similarities

Belongs to the 3-HAO family.

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Product protocols

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Target data

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.
See full target information HAAO
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Primary Antibodies

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Primary Antibodies

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