JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB99387

Recombinant Human HARS protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human HARS protein is a Human Full Length protein, in the 1 to 509 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

HARS, HRS, HARS1, Histidyl-tRNA synthetase, HisRS

2 Images
SDS-PAGE - Recombinant Human HARS protein (AB99387)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human HARS protein (AB99387)

SDS-PAGE analysis of ab99387 (3µg).

SDS-PAGE - Recombinant Human HARS protein (AB99387)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HARS protein (AB99387)

15% SDS-PAGE analysis of 3μg ab99387.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P12081

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC","proteinLength":"Full Length","predictedMolecularWeight":"59.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":509,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P12081","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HARS also known as Histidyl-tRNA synthetase is an enzyme involved in the translation process of protein synthesis. It charges tRNA molecules with histidine facilitating the incorporation of this amino acid into growing polypeptide chains. HARS has an approximate molecular mass of 57 kDa. The enzyme is widely expressed in human tissues including high levels in the liver and skeletal muscle which supports its role in the active protein synthesis associated with these organs.
Biological function summary

Histidyl-tRNA synthetase plays a fundamental role in ensuring accuracy during protein synthesis by attaching histidine to its corresponding tRNA. This enzyme acts as a monomer but often interacts with other synthetases in a multisynthetase complex. By providing histidine-charged tRNAs HARS supports the translation machinery that synthesizes proteins based on encoded genetic instructions reflecting its essentiality in cellular health and function.

Pathways

HARS operates predominantly within the aminoacyl-tRNA biosynthesis pathway which is essential for protein translation. This pathway involves several other aminoacyl-tRNA synthetases including the leucyl-tRNA synthetase (LARS) and lysyl-tRNA synthetase (KARS) which facilitate the attachment of specific amino acids to their respective tRNAs. Additionally HARS links to the broader mTOR signaling pathway a critical pathway for regulating cell growth and protein synthesis in response to nutrient availability.

HARS mutations have association with a range of pathologies particularly autoimmune conditions such as myositis. Changes in HARS expression or function are also observed in neurodegenerative diseases like Charcot-Marie-Tooth disease a disorder characterized by peripheral nerve damage. Connections to these diseases highlight interactions between HARS and proteins involved in immune response and nerve function although the precise mechanisms may require further study.
style
left-column

Specifications

Form

Liquid

Additional notes

ab99387 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP) (PubMed : 29235198). Plays a role in axon guidance (PubMed : 26072516).

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

style
left-column

Product protocols

style
left-column

Target data

Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP) (PubMed : 29235198). Plays a role in axon guidance (PubMed : 26072516).
See full target information HARS1
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB41500

Rabbit Anti-Chicken IgY H&L (15nm Gold)

  • 0
  • 0
Primary Antibodies

AB140640

Anti-HARS antibody [EPR9450(B)(ABC)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HARS antibody [EPR9450(B)(ABC)] (AB140640)

  • 0
  • 0
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com