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AB140544

Recombinant Human HAX1 protein

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Recombinant Human HAX1 protein is a Human Full Length protein, in the 1 to 279 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

HS1BP1, HAX1, HCLS1-associated protein X-1, HS1-associating protein X-1, HS1-binding protein 1, HAX-1, HSP1BP-1

1 Images
SDS-PAGE - Recombinant Human HAX1 protein (AB140544)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HAX1 protein (AB140544)

15% SDS-PAGE analysis of ab140544 (3μg).

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

O00165

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSLFDLFRGFFGFPGPRSHRDPFFGGMTRDEDDDEEEEEEGGSWGRGNPRFHSPQHPPEEFGFGFSFSPGGGIRFHDNFGFDDLVRDFNSIFSDMGAWTLPSHPPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFGGVLESDARSESPQPAPDWGSQRPFHRFDDVWPMDPHPRTREDNDLDSQVSQEGLGPVLQPQPKSYFKSISVTKITKPDGIVEERRTVVDSEGRTETTVTRHEADSSPRGDPESPRPPALDDAFSILDLFLGRWFRSR","proteinLength":"Full Length","predictedMolecularWeight":"33.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":279,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00165","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HAX1 also known as HS-1 associated protein X-1 is a multifunctional protein with a mass of approximately 35 kDa. It plays a significant role in cellular activities by regulating various signaling pathways and maintaining cellular homeostasis. The expression of HAX1 is found in several tissues including the brain heart and skeletal muscle indicating its diverse functional importance across different cell types.
Biological function summary

HAX1 contributes to the stabilization of mitochondria by preventing apoptosis. It interacts with other proteins to form complexes that support cell survival. HAX1's involvement in mitochondrial functions highlights its role in regulating energy production and cellular stress responses. Its capacity to inhibit programmed cell death makes it essential for maintaining cell integrity under stress conditions.

Pathways

HAX1 is integral to the mitochondrial apoptosis pathway and also plays a part in the cytoskeletal signaling pathway. In the apoptosis pathway HAX1 interacts with proteins like BCL-2 to inhibit cell death ensuring cell survival. Additionally in the cytoskeletal signaling pathway HAX1 associates with cytoskeletal proteins aiding in the reorganization of cell structure and enhancing cellular processes like migration.

HAX1 mutations or dysregulation link to severe congenital neutropenia and some neurological disorders. Mutations in HAX1 disrupt the apoptosis inhibition pathway causing a loss in neutrophil count which results in severe congenital neutropenia. This condition is often associated with proteins like ELANE. In some neurological disorders altered HAX1 levels can disturb cellular signaling and survival leading to cellular dysfunction.
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Specifications

Form

Liquid

Additional notes

ab140544 is purified using conventional chromatography techniques.

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General info

Function

Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex (PubMed : 26997484). Slows down the rate of inactivation of KCNC3 channels (PubMed : 26997484). Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.

Sequence similarities

Belongs to the HAX1 family.

Post-translational modifications

Proteolytically cleaved by caspase-3 during apoptosis.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex (PubMed : 26997484). Slows down the rate of inactivation of KCNC3 channels (PubMed : 26997484). Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.
See full target information HAX1
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Complementary Products

Primary Antibodies

AB137613

Anti-HAX1 antibody

Western blot - Anti-HAX1 antibody (AB137613)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104

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