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AB310795

Recombinant Human HBEGF/DRT Protein

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Recombinant Human HBEGF/DRT Protein is a Human Fragment protein, in the 63 to 148 aa range, expressed in HEK 293 cells, with >95%, >0.005 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, Mass Spec.

View Alternative Names

DTR, DTS, HEGFL, HBEGF, Proheparin-binding EGF-like growth factor

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Mass Spectrometry - Recombinant Human HBEGF/DRT Protein (AB310795)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human HBEGF/DRT Protein (AB310795)

Mass determination by ESI-TOF. Predicted MW is 9786.5 Da (+/-10 Da by ESI-TOF). Observed MW is 9786.19 Da.

SDS-PAGE - Recombinant Human HBEGF/DRT Protein (AB310795)
  • SDS-PAGE

Lab

SDS-PAGE - Recombinant Human HBEGF/DRT Protein (AB310795)

SDS-PAGE analysis of ab310795

HPLC - Recombinant Human HBEGF/DRT Protein (AB310795)
  • HPLC

Supplier Data

HPLC - Recombinant Human HBEGF/DRT Protein (AB310795)

HPLC analysis of ab310795

Key facts

Purity

>95% HPLC

SDS-PAGE>=95%

Endotoxin level

>0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, HPLC, Mass Spec

applications

Biologically active

No

Accession

Q99075

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

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Recombinant human FGF2 protein (Active)

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Primary Antibodies

AB218019

Anti-HBEGF/DTR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HBEGF/DTR antibody (AB218019)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"DLQEADLDLLRVTLSSKPQALATPNKEEHGKRKKKGKGLGKKRDPCLRKYKDFCIHGECKYVKELRAPSCICHPGYHGERCHGLSL","proteinLength":"Fragment","predictedMolecularWeight":"9.79 kDa","actualMolecularWeight":"9.79 kDa","aminoAcidEnd":148,"aminoAcidStart":63,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q99075","tags":[]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HBEGF also known as Heparin-binding EGF-like growth factor or Diphtheria Toxin Receptor (DTR) is a growth factor that binds to EGF receptors with its mass approximately 20 kilodaltons. It is a member of the EGF family. Cells secrete HBEGF and it is expressed in many tissues including the heart kidney and lungs. Its expression often increases during proliferation and differentiation making it an important player in tissue growth and repair.
Biological function summary

HBEGF functions as a mitogen and chemotactic agent which means it stimulates cell division and attracts cells to migrate. As part of the EGF family HBEGF activates the EGFR (epidermal growth factor receptor) signaling cascade. This signaling is essential for processes such as wound healing and development. HBEGF also forms complexes with other ligands and receptors enhancing its biological activity in tissues.

Pathways

HBEGF plays a role in the MAPK/ERK signaling pathway and the PI3K/AKT pathway. Both pathways are significant for regulating cell growth survival and differentiation. HBEGF interacts with other proteins like Ras and PI3K in these pathways. By activating these pathways HBEGF influences cellular responses critical for normal physiological processes and recovery mechanisms in cells.

HBEGF has connections with cancer and cardiovascular diseases. Aberrant HBEGF signaling can promote oncogenic processes in cancer relating it to proteins such as HER2 which also engage in similar pathways. In cardiovascular diseases HBEGF overexpression can contribute to smooth muscle cell proliferation an important event in atherosclerosis linking it to other growth factors involved in vascular biology.
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General info

Function

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Post-translational modifications

Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.. O-glycosylated with core 1 or possibly core 8 glycans. Thr-47 is a minor glycosylation site compared to Thr-44.

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Product protocols

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Target data

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
See full target information HBEGF
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Product promise

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