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AB205523

Recombinant human HBEGF/DTR protein (Animal Free)

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(1 Publication)

Recombinant human HBEGF/DTR protein (Animal Free) is a Human Full Length protein, in the 63 to 148 aa range, expressed in Escherichia coli, with >97%, < 0.05 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

DTR, DTS, HEGFL, HBEGF, Proheparin-binding EGF-like growth factor

Key facts

Purity

>97% SDS-PAGE

Endotoxin level

< 0.05 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The activity is determined by the ability to induce proliferation of 3T3 cells and is typically less than 0.2 ng/mL. This corresponds to an expected specific activity of 5 x 106 units/mg.

Accession

Q99075

Animal free

Yes

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.16% Sodium phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as HB EGF
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Sequence info

[{"sequence":"DLQEADLDLLRVTLSSKPQALATPNKEEHGKRKKKGKGLGKKRDPCLRKYKDFCIHGECKYVKELRAPSCICHPGYHGERCHGLSL","proteinLength":"Full Length","predictedMolecularWeight":"9.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":148,"aminoAcidStart":63,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q99075","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon reconstitution add a carrier protein (0.1% BSA)
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HBEGF also known as Heparin-binding EGF-like growth factor or Diphtheria Toxin Receptor (DTR) is a growth factor that binds to EGF receptors with its mass approximately 20 kilodaltons. It is a member of the EGF family. Cells secrete HBEGF and it is expressed in many tissues including the heart kidney and lungs. Its expression often increases during proliferation and differentiation making it an important player in tissue growth and repair.
Biological function summary

HBEGF functions as a mitogen and chemotactic agent which means it stimulates cell division and attracts cells to migrate. As part of the EGF family HBEGF activates the EGFR (epidermal growth factor receptor) signaling cascade. This signaling is essential for processes such as wound healing and development. HBEGF also forms complexes with other ligands and receptors enhancing its biological activity in tissues.

Pathways

HBEGF plays a role in the MAPK/ERK signaling pathway and the PI3K/AKT pathway. Both pathways are significant for regulating cell growth survival and differentiation. HBEGF interacts with other proteins like Ras and PI3K in these pathways. By activating these pathways HBEGF influences cellular responses critical for normal physiological processes and recovery mechanisms in cells.

HBEGF has connections with cancer and cardiovascular diseases. Aberrant HBEGF signaling can promote oncogenic processes in cancer relating it to proteins such as HER2 which also engage in similar pathways. In cardiovascular diseases HBEGF overexpression can contribute to smooth muscle cell proliferation an important event in atherosclerosis linking it to other growth factors involved in vascular biology.
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Specifications

Form

Lyophilized

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General info

Function

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Post-translational modifications

Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.. O-glycosylated with core 1 or possibly core 8 glycans. Thr-47 is a minor glycosylation site compared to Thr-44.

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Product protocols

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Target data

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
See full target information HBEGF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell research 30:779-793 PubMed32296111

2020

s-HBEGF/SIRT1 circuit-dictated crosstalk between vascular endothelial cells and keratinocytes mediates sorafenib-induced hand-foot skin reaction that can be reversed by nicotinamide.

Applications

Unspecified application

Species

Unspecified reactive species

Peihua Luo,Hao Yan,Xueqin Chen,Ying Zhang,Ziying Zhao,Ji Cao,Yi Zhu,Jiangxia Du,Zhifei Xu,Xiaochen Zhang,Su Zeng,Bo Yang,Shenglin Ma,Qiaojun He
View all publications
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