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AB183246

Recombinant Human HCE protein (His tag N-Terminus)

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Recombinant Human HCE protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 597 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

CAP1A, RNGTT, mRNA-capping enzyme, HCAP1, HCE

1 Images
SDS-PAGE - Recombinant Human HCE protein (His tag N-Terminus) (AB183246)
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Supplier Data

SDS-PAGE - Recombinant Human HCE protein (His tag N-Terminus) (AB183246)

15% SDS-PAGE analysis of ab183246 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O60942

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAHNKIPPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKVKMGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERNPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPLLPDWCFEDDEDEDEDEDGKKESEPGSSASFGKRRKERLKLGAIFLEGVTVKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGKYKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVTKELKQYDNKIIECKFENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCTAASQGQKRKHHLDPDTELMPPPPPKRPRPLT","proteinLength":"Full Length","predictedMolecularWeight":"70.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":597,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O60942","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HCE also known as Heat Shock Cognate 70 is a stress-responsive protein with a mass of approximately 70 kDa. This protein plays a mechanical role as a molecular chaperone assisting in the proper folding of nascent polypeptides and the refolding of misfolded proteins. HCE expression occurs in various tissues but notably it is abundant in the cytoplasm of cells exposed to stressful conditions like heat shock. The protein assists in maintaining cellular protein homeostasis particularly during periods of increased environmental stress.
Biological function summary

HCE contributes to multiple cellular processes critical for survival and adaptation. It is an important component of the chaperone complex and interacts with co-chaperones to facilitate protein folding and prevent aggregation. HCE enhances cellular resilience by stabilizing proteins and protecting against cellular stress-induced damage. Additionally it supports proteostasis by targeting misfolded proteins for degradation via the ubiquitin-proteasome system.

Pathways

HCE is involved in essential cellular mechanisms beyond protein folding including the unfolded protein response (UPR) and heat shock response (HSR). In these pathways HCE collaborates with proteins like HSP40 and HSP90 to restore normal protein conformation and function. The heat shock response pathway is a protective cellular mechanism that activates a cascade to manage stress-related protein damage and ensure cell survival during acute stress.

HCE has significant connections to neurodegenerative diseases such as Alzheimer’s and Parkinson’s. Research indicates that aberrant HCE function may contribute to protein aggregation a hallmark of these conditions. In Alzheimer’s disease HCE interacts with proteins like tau and amyloid precursor protein (APP) influencing pathological aggregation processes. Understanding the role of HCE in these disorders opens potential avenues for therapeutic targeting to mitigate protein aggregation and fibrous plaques characteristic of neurodegeneration.
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Specifications

Form

Liquid

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General info

Function

Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphate monophosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation : by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate.

Sequence similarities

In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.. In the C-terminal section; belongs to the eukaryotic GTase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphate monophosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation : by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate.
See full target information RNGTT
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