Recombinant human Hck protein
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant human Hck protein is a Human Fragment protein, in the 230 to 497 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
View Alternative Names
Tyrosine-protein kinase HCK, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck, HCK
- FuncS
Supplier Data
Functional Studies - Recombinant human Hck protein (AB60865)
The specific activity of Hck (ab60865) was determined to be 290 nmol /min/mg as per activity assay protocol.
- FuncS
Unknown
Functional Studies - Recombinant human Hck protein (AB60865)
Sample Kinase Activity Plot.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human Hck protein (AB60865)
ab60865 on SDS-PAGE, MW ~57 kDa.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human Hck protein (AB60865)
SDS PAGE analysis of ab60865
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Hck is involved in immune system regulation and cell signaling processes. It operates within complexes that facilitate its kinase activity and substrate specificity. Hck influences pathways that regulate immune cell responses like phagocytosis and cell migration. It helps in cellular adhesion and the production of reactive oxygen species both of which are vital for the proper functioning of myeloid cells in the immune response.
Pathways
Several signaling processes use Hck for transmitting signals from the outside of the cell to the inside. Hck participates in the Fc gamma receptor-mediated phagocytosis pathway and the chemokine signaling pathway. In these pathways Hck collaborates with proteins such as Src and Lyn enhancing the immune response and cellular communication. These pathways control important immune functions and cellular responses.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Post-translational modifications
Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases.. Ubiquitinated by CBL, leading to its degradation via the proteasome.. Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2.
Subcellular localisation
Lysosome
Target data
Complementary Products
![Western blot - Anti-Hck antibody [EP2415Y] (AB75839)](https://content.abcam.com/products/images/hck-antibody-ep2415y-ab75839--western-blot-img402571.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com