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AB108376

Recombinant Human HCM protein

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Recombinant Human HCM protein is a Human Full Length protein, in the 1 to 476 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

KIAA0398, RNMT, mRNA cap guanine-N(7) methyltransferase, RG7MT1, mRNA (guanine-N(7))-methyltransferase, mRNA cap methyltransferase, hCMT1, hMet, hcm1p

1 Images
SDS-PAGE - Recombinant Human HCM protein (AB108376)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human HCM protein (AB108376)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O43148

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMANSAKAEEYEKMSLEQAKASVNSETESSFNINENTTASGTGLSEKTSVCRQVDIARKRKEFEDDLVKESSSCGKDTPSKKRKLDPEIVPEEKDCGDAEGNSKKRKRETEDVPKDKSSTGDGTQNKRKIALEDVPEKQKNLEEGHSSTVAAHYNELQEVGLEKRSQSRIFYLRNFNNWMKSVLIGEFLEKVRQKKKRDITVLDLGCGKGGDLLKWKKGRINKLVCTDIADVSVKQCQQRYEDMKNRRDSEYIFSAEFITADSSKELLIDKFRDPQMCFDICSCQFVCHYSFESYEQADMMLRNACERLSPGGYFIGTTPNSFELIRRLEASETESFGNEIYTVKFQKKGDYPLFGCKYDFNLEGVVDVPEFLVYFPLLNEMAKKYNMKLVYKKTFLEFYEEKIKNNENKMLLKRMQALEPYPANESSKLVSEKVDDYEHAAKYMKNSQVRLPLGTLSKSEWEATSIYLVFAFEKQQ","proteinLength":"Full Length","predictedMolecularWeight":"57 kDa","actualMolecularWeight":null,"aminoAcidEnd":476,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O43148","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target HCM also known as Heart-Type Fatty Acid-Binding Protein plays a critical mechanical role in binding and transporting long-chain fatty acids. It has a molecular mass of approximately 14-15 kDa. HCM is found mainly in cytoplasm of cardiac tissue although small amounts can be seen in skeletal muscle and kidneys. The protein acts as a carrier within the cell facilitating the availability of fatty acids for energy metabolism and signaling.
Biological function summary

Heart-Type Fatty Acid-Binding Protein supports energy homeostasis in heart cells by aiding in the uptake and utilization of fatty acids. This protein often functions as part of larger complexes involved in metabolism and cellular transport. By managing fatty acid distribution within cells HCM contributes significantly to the energy supply especially during increased cardiac workload.

Pathways

The activity of Heart-Type Fatty Acid-Binding Protein integrates into critical metabolic pathways including lipid metabolism and fatty acid oxidation. Within these pathways HCM works alongside enzymes like acyl-CoA synthetase which activates fatty acids to promote efficient metabolic processes. The coordinated actions of these proteins ensure proper energy balance and cellular health.

HCM is notably connected to cardiac conditions such as myocardial infarction. Elevated levels of this protein in blood serve as a biomarker for acute heart injury. The relationship between HCM and disorders like heart failure links to its role alongside proteins like creatine kinase which also rises in cardiac stress. These connections make HCM essential in diagnostic approaches for heart diseases.
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Specifications

Form

Liquid

Additional notes

ab108376 is purified using conventional chromatography techniques.

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General info

Function

Catalytic subunit of the mRNA-capping methyltransferase RNMT : RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs (PubMed : 10347220, PubMed : 11114884, PubMed : 22099306, PubMed : 27422871, PubMed : 9705270, PubMed : 9790902). Binds RNA containing 5'-terminal GpppC (PubMed : 11114884).

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalytic subunit of the mRNA-capping methyltransferase RNMT : RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs (PubMed : 10347220, PubMed : 11114884, PubMed : 22099306, PubMed : 27422871, PubMed : 9705270, PubMed : 9790902). Binds RNA containing 5'-terminal GpppC (PubMed : 11114884).
See full target information RNMT
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