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AB268611

Recombinant human HDAC10 protein (Active)

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Recombinant human HDAC10 protein (Active) is a Human Fragment protein, in the 1 to 482 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Polyamine deacetylase HDAC10, Histone deacetylase 10, HD10, HDAC10

2 Images
Functional Studies - Recombinant human HDAC10 protein (Active) (AB268611)
  • FuncS

Supplier Data

Functional Studies - Recombinant human HDAC10 protein (Active) (AB268611)

The specific activity of HDAC10 was determined to be 110 RLU/min/ng HDAC-Glo assay using HDAC10 substrate.

SDS-PAGE - Recombinant human HDAC10 protein (Active) (AB268611)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human HDAC10 protein (Active) (AB268611)

SDS-PAGE analysis of ab268611.

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of HDAC10 was determined to be 110 RLU/min/ng HDAC-Glo assay using HDAC10 substrate.

Accession

Q969S8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGTALVYHEDMTATRLLWDDPECEIERPERLTAALDRLRQRGLEQRCLRLSAREASEEELGLVHSPEYVSLVRETQVLGKEELQALSGQFDAIYFHPSTFHCARLAAGAGLQLVDAVLTGAVQNGLALVRPPGHHGQRAAANGFCVFNNVAIAAAHAKQKHGLHRILVVDWDVHHGQGIQYLFEDDPSVLYFSWHRYEHGRFWPFLRESDADAVGRGQGLGFTVNLPWNQVGMGNADYVAAFLHLLLPLAFEFDPELVLVSAGFDSAIGDPEGQMQATPECFAHLTQLLQVLAGGRVCAVLEGGYHLESLAESVCMTVQTLLGDPAPPLSGPMAPCQSALESIQSARAAQAPHWKSLQQQDVTAVPMSPSSHSPEGRPPPLLPGGPVCKAAASAPSSLLDQPCLCPAPSVRTAVALTTPDITLVLPPDVIQQEASALREETEAWARPHESLAREEALTALGKLLYLLDGMLDGQVNSGIAAT","proteinLength":"Fragment","predictedMolecularWeight":"71 kDa","actualMolecularWeight":null,"aminoAcidEnd":482,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q969S8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC10 also known as Histone Deacetylase 10 is an enzyme belonging to the HDAC family instrumental in removing acetyl groups from histone proteins and other non-histone proteins. This protein around 71 kDa in mass influences chromatin structure and regulates gene transcription. HDAC10 is expressed in various tissues including the kidney and liver and plays a direct role in the modulation of gene expression through its deacetylase activity impacting cellular processes and environmental responses.
Biological function summary

HDAC10 functions as a regulatory component within the cellular milieu influencing transcriptional repression across several pathways. HDAC10 often forms part of multi-protein complexes although not extensively characterized they include other HDAC family members. It participates in controlling cell growth and cell cycle progress and shows involvement in DNA repair mechanisms. By modulating gene expression through deacetylation HDAC10 impacts cellular proliferation and differentiation.

Pathways

HDAC10 plays a role in the PI3K/AKT signaling pathway which is important for cell survival and growth. HDAC10 interacts with proteins like AKT1 assisting with the transduction of the signaling cascade that contributes to cell cycle progression and apoptosis regulation. Also this enzyme interplays with the autophagy pathway influencing cellular metabolism and homeostasis through its deacetylation functions impacting Beclin-1 regulation and activity levels.

HDAC10 has been implicated in cancer and neurodegenerative diseases. In cancer particularly renal carcinoma HDAC10 may alter oncogene expression and tumor-suppressor levels connecting with proteins such as p53 which are vital in cell-cycle regulation and apoptosis. In neurodegenerative disorders modulations in HDAC10 levels affect huntingtin protein acetylation impacting the progression of diseases like Huntington's disease. Its role in histone and non-histone protein regulation positions HDAC10 as a potential target for therapeutic interventions in these conditions.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Polyamine deacetylase (PDAC), which acts preferentially on N(8)-acetylspermidine, and also on acetylcadaverine and acetylputrescine (PubMed : 28516954). Exhibits attenuated catalytic activity toward N(1),N(8)-diacetylspermidine and very low activity, if any, toward N(1)-acetylspermidine (PubMed : 28516954). Histone deacetylase activity has been observed in vitro (PubMed : 11677242, PubMed : 11726666, PubMed : 11739383, PubMed : 11861901). Has also been shown to be involved in MSH2 deacetylation (PubMed : 26221039). The physiological relevance of protein/histone deacetylase activity is unclear and could be very weak (PubMed : 28516954). May play a role in the promotion of late stages of autophagy, possibly autophagosome-lysosome fusion and/or lysosomal exocytosis in neuroblastoma cells (PubMed : 23801752, PubMed : 29968769). May play a role in homologous recombination (PubMed : 21247901). May promote DNA mismatch repair (PubMed : 26221039).

Sequence similarities

Belongs to the histone deacetylase family. HD type 2 subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Polyamine deacetylase (PDAC), which acts preferentially on N(8)-acetylspermidine, and also on acetylcadaverine and acetylputrescine (PubMed : 28516954). Exhibits attenuated catalytic activity toward N(1),N(8)-diacetylspermidine and very low activity, if any, toward N(1)-acetylspermidine (PubMed : 28516954). Histone deacetylase activity has been observed in vitro (PubMed : 11677242, PubMed : 11726666, PubMed : 11739383, PubMed : 11861901). Has also been shown to be involved in MSH2 deacetylation (PubMed : 26221039). The physiological relevance of protein/histone deacetylase activity is unclear and could be very weak (PubMed : 28516954). May play a role in the promotion of late stages of autophagy, possibly autophagosome-lysosome fusion and/or lysosomal exocytosis in neuroblastoma cells (PubMed : 23801752, PubMed : 29968769). May play a role in homologous recombination (PubMed : 21247901). May promote DNA mismatch repair (PubMed : 26221039).
See full target information HDAC10
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