Recombinant human HDAC4 protein
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(2 Publications)
Recombinant human HDAC4 protein is a Human Fragment protein, in the 612 to 1084 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.
View Alternative Names
KIAA0288, HDAC4, Histone deacetylase 4, HD4
- FuncS
Unknown
Functional Studies - Recombinant human HDAC4 protein (AB104029)
Kinase Assay demonstrating specific activity of ab104029.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human HDAC4 protein (AB104029)
SDS-PAGE showing ab104029 at approximately 77kDa.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.
Pathways
HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.
Specifications
Form
Liquid
Additional notes
Purity was determined to be >95% by densitometry. Affinity purified.
General info
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Post-translational modifications
Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350, within the PxLPxI/L motif, impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3.. Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
Subcellular localisation
Nucleus
Target data
Publications (2)
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The Prostate 83:1470-1493 PubMed37559436
2023
Applications
Unspecified application
Species
Unspecified reactive species
Journal of molecular cell biology 11:158-169 PubMed29800227
2018
Applications
Unspecified application
Species
Unspecified reactive species
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