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AB268614

Recombinant human HDAC4 protein (Active)

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Recombinant human HDAC4 protein (Active) is a Human Fragment protein, in the 101 to 1084 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA0288, HDAC4, Histone deacetylase 4, HD4

2 Images
Functional Studies - Recombinant human HDAC4 protein (Active) (AB268614)
  • FuncS

Supplier Data

Functional Studies - Recombinant human HDAC4 protein (Active) (AB268614)

The specific activity of HDAC4 was determined to be 80 RLU/min/ng in a HDAC-Glo assay using HDAC4 substrate.

SDS-PAGE - Recombinant human HDAC4 protein (Active) (AB268614)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human HDAC4 protein (Active) (AB268614)

SDS-PAGE analysis of ab268614.

MW ~155 kDa.

Key facts

Purity

>70% SDS-PAGE

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of HDAC4 was determined to be 80 RLU/min/ng in a HDAC-Glo assay using HDAC4 substrate.

Accession

P56524

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Complementary Products

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Proteins & Peptides

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Assay Kits

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"SRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQKLQQLKNKEKGKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISSDPRYWYGKTQHSSLDQSSPPQSGVSTSYNHPVLGMYDAKDDFPLRKTASEPNLKLRSRLKQKVAERRSSPLLRRKDGPVVTALKKRPLDVTDSACSSAPGSGPSSPNNSSGSVSAENGIAPAVPSIPAETSLAHRLVAREGSAAPLPLYTSPSLPNITLGLPATGPSAGTAGQQDAERLTLPALQQRLSLFPGTHLTPYLSTSPLERDGGAAHSPLLQHMVLLEQPPAQAPLVTGLGALPLHAQSLVGADRVSPSIHKLRQHRPLGRTQSAPLPQNAQALQHLVIQQQHQQFLEKHKQQFQQQQLQMNKIIPKPSEPARQPESHPEETEEELREHQALLDEPYLDRLPGQKEAHAQAGVQVKQEPIESDEEEAEPPREVEPGQRQPSEQELLFRQQALLLEQQRIHQLRNYQASMEAAGIPVSFGGHRPLSRAQSSPASATFPVSVQEPPTKPRFTTGLVYDTLMLKHQCTCGSSSSHPEHAGRIQSIWSRLQETGLRGKCECIRGRKATLEELQTVHSEAHTLLYGTNPLNRQKLDSKKLLGSLASVFVRLPCGGVGVDSDTIWNEVHSAGAARLAVGCVVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWDVHHGNGTQQAFYSDPSVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLDPPMGDAEYLAAFRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSARCFGYLTKQLMGLAGGRIVLALEGGHDLTAICDASEACVSALLGNELDPLPEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGRSLIEAQTCENEEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1084,"aminoAcidStart":101,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P56524","tags":[{"tag":"GST","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC4 also known as Histone Deacetylase 4 functions in the regulation of gene expression through chromatin remodeling. This protein removes acetyl groups from histone proteins causing chromatin compaction and transcriptional repression. HDAC4 has a molecular weight of approximately 140 kDa. The protein is found in various tissues including brain heart and skeletal muscle and plays essential roles in various cellular processes. Alternate names for HDAC4 include KIAA0288 and HD4.
Biological function summary

The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.

Pathways

HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.

HDAC4 has associations with neurological disorders and cancer. Mutations and dysregulation in HDAC4 are linked to neuronal disorders such as Huntington's disease. In cancer HDAC4 interacts with other oncogenic proteins such as p53 influencing tumor progression and resistance to therapy. Consequently HDAC4 presents as a potential target for therapeutic intervention in these disease contexts.
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General info

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).

Sequence similarities

Belongs to the histone deacetylase family. HD type 2 subfamily.

Post-translational modifications

Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350, within the PxLPxI/L motif, impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3.. Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).
See full target information HDAC4
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