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AB80348

Recombinant human HDAC5 protein

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Recombinant human HDAC5 protein is a Human Full Length protein, in the 1 to 1122 aa range, expressed in Baculovirus, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA0600, HDAC5, Histone deacetylase 5, HD5, Antigen NY-CO-9

2 Images
Functional Studies - Recombinant human HDAC5 protein (AB80348)
  • FuncS

Unknown

Functional Studies - Recombinant human HDAC5 protein (AB80348)

Image showing specific activity of ab80348.

SDS-PAGE - Recombinant human HDAC5 protein (AB80348)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human HDAC5 protein (AB80348)

10% SDS-PAGE showing ab80348 at approximately 150kDa (7μg).

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥2500 pmol/min/μg.

Unit Definition: One U =1 pmol of acetyl group removed/min/μg of enzyme.

Assay Conditions:

25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM substrate, and 0.8-50 ng/μl HDAC5. Incubation condition: 30 min at 37°C followed by HDAC developer for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

Accession

Q9UQL6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.8004% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1122,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UQL6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC5 also known as histone deacetylase 5 is an enzyme that removes acetyl groups from histone proteins leading to alteration of chromosome structure and regulation of gene expression. Its molecular weight is approximately 122 kDa. HDAC5 belongs to the class IIa HDAC family and shows high expression in heart brain and skeletal muscle. This expression pattern suggests its importance in these tissues. The role of HDAC5 in dynamically regulating chromatin structure positions it as an important modulator in the tight control of gene transcription.
Biological function summary

HDAC5 plays a pivotal role in gene silencing and is a member of multi-protein complexes. It often partners with other HDACs and co-repressor proteins to enforce transcriptional repression. Through its activity HDAC5 affects cellular processes such as cell growth differentiation and apoptosis. Its expression and activity levels fluctuate among different cell types reflecting its involvement in tissue-specific functions and responses to cellular cues.

Pathways

HDAC5 participates in critical signaling cascades like the MEF2 and NF-kB pathways. In the MEF2 pathway HDAC5 interacts closely with the MEF2 transcription factors repressing genes involved in muscle differentiation. Within the NF-kB pathway HDAC5 contributes to the regulation of inflammatory responses by modulating gene expression. The intricate relationship between HDAC5 and these pathways highlights its regulative influence on cellular functions and adaptive responses.

Recent research implicates HDAC5 in cancer and cardiac hypertrophy. Its overactivity or dysregulation has been observed in certain cancers where it may aid tumor progression by promoting oncogenic gene expression. Similarly in cardiac hypertrophy HDAC5 dysregulation can lead to maladaptive heart muscle growth a condition often exacerbated through interactions with protein kinases like CaMK. These associations have spurred interest in developing HDAC5 inhibitors as potential therapeutic targets to modulate its activity and address these diseases.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758).

Sequence similarities

Belongs to the histone deacetylase family. HD type 2 subfamily.

Post-translational modifications

Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription (By similarity).. Ubiquitinated. Polyubiquitination however does not lead to its degradation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758).
See full target information HDAC5
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