Recombinant human HDAC6 protein (Active) is a Human Full Length protein, in the 1 to 1215 aa range, expressed in Baculovirus infected Sf9, with >65% purity and suitable for SDS-PAGE, FuncS, Inhib.
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Publications
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- Journal of medicinal chemistry 61:6056-60742018Discovery of Janus Kinase 2 (JAK2) and Histone Deacetylase (HDAC) Dual Inhibitors as a Novel Strategy for the Combinational Treatment of Leukemia and Invasive Fungal Infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYahui Huang et. al.PubMed 29940115
- European journal of medicinal chemistry 141:596-6022017Discovery of a fluorescent probe with HDAC6 selective inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingjie Zhang et. al.PubMed 29102179
- Journal of medicinal chemistry 58:4325-382015Design, synthesis, and antitumor evaluation of novel histone deacetylase inhibitors equipped with a phenylsulfonylfuroxan module as a nitric oxide donor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenwen Duan et. al.PubMed 25906087
Key facts
- Purity
- >65% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS, Inhib
- Biologically active
- Yes
Amino acid sequence
" M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D P M G H H H H H H G R R R A S V A A G I L V P R G S P G L D G I Y A R G I Q M T S T G Q D S T T T R Q R R S R Q N P Q S P P Q D S S V T S K R N I K K G A V P R S I P N L A E V K K K G K M K K L G Q A M E E D L I V G L Q G M D L N L E A E A L A G T G L V L D E Q L N E F H C L W D D S F P E G P E R L H A I K E Q L I Q E G L L D R C V S F Q A R F A E K E E L M L V H S L E Y I D L M E T T Q Y M N E G E L R V L A D T Y D S V Y L H P N S Y S C A C L A S G S V L R L V D A V L G A E I R N G M A I I R P P G H H A Q H S L M D G Y C M F N H V A V A A R Y A Q Q K H R I R R V L I V D W D V H H G Q G T Q F T F D Q D P S V L Y F S I H R Y E Q G R F W P H L K A S N W S T T G F G Q G Q G Y T I N V P W N Q V G M R D A D Y I A A F L H V L L P V A L E F Q P Q L V L V A A G F D A L Q G D P K G E M A A T P A G F A Q L T H L L M G L A G G K L I L S L E G G Y N L R A L A E G V S A S L H T L L G D P C P M L E S P G A P C R S A Q A S V S C A L E A L E P F W E V L V R S T E T V E R D N M E E D N V E E S E E E G P W E P P V L P I L T W P V L Q S R T G L V Y D Q N M M N H C N L W D S H H P E V P Q R I L R I M C R L E E L G L A G R C L T L T P R P A T E A E L L T C H S A E Y V G H L R A T E K M K T R E L H R E S S N F D S I Y I C P S T F A C A Q L A T G A A C R L V E A V L S G E V L N G A A V V R P P G H H A E Q D A A C G F C F F N S V A V A A R H A Q T I S G H A L R I L I V D W D V H H G N G T Q H M F E D D P S V L Y V S L H R Y D H G T F F P M G D E G A S S Q I G R A A G T G F T V N V A W N G P R M G D A D Y L A A W H R L V L P I A Y E F N P E L V L V S A G F D A A R G D P L G G C Q V S P E G Y A H L T H L L M G L A S G R I I L I L E G G Y N L T S I S E S M A A C T R S L L G D P P P L L T L P R P P L S G A L A S I T E T I Q V H R R Y W R S L R V M K V E D R E G P S S S K L V T K K A P Q P A K P R L A E R M T T R E K K V L E A G M G K V T S A S F G E E S T P G Q T N S E T A V V A L T Q D Q P S E A A T G G A T L A Q T I S E A A I G G A M L G Q T T S E E A V G G A T P D Q T T S E E T V G G A I L D Q T T S E D A V G G A T L G Q T T S E E A V G G A T L A Q T T S E A A M E G A T L D Q T T S E E A P G G T E L I Q T P L A S S T D H Q T P P T S P V Q G T T P Q I S P S T L I G S L R T L E L G S E S Q G A S E S Q A P G E E N L L G E A A G G Q D M A D S M L M Q G S R G L T D Q A I F Y A V T P L P W C P H L V A V C P I P A A G L D V T Q P C G D C G T I Q E N W V C L S C Y Q V Y C G R Y I N G H M L Q H H G N S G H P L V L S Y I D L S A W C Y Y C Q A Y V H H Q A L L D V K N I A H Q N K F G E D M P H P H "
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application Inhib | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Associated Products
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Target data
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10220385). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10220385). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10220385). In addition to histones, deacetylates other proteins, such as CTTN, tubulin and SQSTM1 (PubMed:12024216, PubMed:20308065, PubMed:26246421, PubMed:30538141, PubMed:31857589). Plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin (PubMed:12024216, PubMed:20308065, PubMed:26246421). Required for cilia disassembly; via deacetylation of alpha-tubulin (PubMed:17604723, PubMed:26246421). Promotes deacetylation of CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy (PubMed:30538141). Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer (PubMed:24413532). Promotes odontoblast differentiation following IPO7-mediated nuclear import and subsequent repression of RUNX2 expression (By similarity). In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome (PubMed:17846173). Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy (PubMed:17846173).
Alternative names
KIAA0901, JM21, HDAC6, Histone deacetylase 6, HD6, Protein deacetylase HDAC6, Tubulin-lysine deacetylase HDAC6
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Recombinant human HDAC6 protein (Active) is a Human Full Length protein, in the 1 to 1215 aa range, expressed in Baculovirus infected Sf9, with >65% purity and suitable for SDS-PAGE, FuncS, Inhib.
Key facts
- Purity
- >65% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS, Inhib
- Biologically active
- Yes
- Biological activity
- Specific Activity: ≥350 pmol/min/μg.Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 3, and HDAC6 (3.1 – 200 ng). Incubation condition: 30 min at 37°C followed by developing for 15 min at room temperature.
- Accession
- Q9UBN7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.0165% Potassium chloride
Sequence info
Amino acid sequence
- " M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D P M G H H H H H H G R R R A S V A A G I L V P R G S P G L D G I Y A R G I Q M T S T G Q D S T T T R Q R R S R Q N P Q S P P Q D S S V T S K R N I K K G A V P R S I P N L A E V K K K G K M K K L G Q A M E E D L I V G L Q G M D L N L E A E A L A G T G L V L D E Q L N E F H C L W D D S F P E G P E R L H A I K E Q L I Q E G L L D R C V S F Q A R F A E K E E L M L V H S L E Y I D L M E T T Q Y M N E G E L R V L A D T Y D S V Y L H P N S Y S C A C L A S G S V L R L V D A V L G A E I R N G M A I I R P P G H H A Q H S L M D G Y C M F N H V A V A A R Y A Q Q K H R I R R V L I V D W D V H H G Q G T Q F T F D Q D P S V L Y F S I H R Y E Q G R F W P H L K A S N W S T T G F G Q G Q G Y T I N V P W N Q V G M R D A D Y I A A F L H V L L P V A L E F Q P Q L V L V A A G F D A L Q G D P K G E M A A T P A G F A Q L T H L L M G L A G G K L I L S L E G G Y N L R A L A E G V S A S L H T L L G D P C P M L E S P G A P C R S A Q A S V S C A L E A L E P F W E V L V R S T E T V E R D N M E E D N V E E S E E E G P W E P P V L P I L T W P V L Q S R T G L V Y D Q N M M N H C N L W D S H H P E V P Q R I L R I M C R L E E L G L A G R C L T L T P R P A T E A E L L T C H S A E Y V G H L R A T E K M K T R E L H R E S S N F D S I Y I C P S T F A C A Q L A T G A A C R L V E A V L S G E V L N G A A V V R P P G H H A E Q D A A C G F C F F N S V A V A A R H A Q T I S G H A L R I L I V D W D V H H G N G T Q H M F E D D P S V L Y V S L H R Y D H G T F F P M G D E G A S S Q I G R A A G T G F T V N V A W N G P R M G D A D Y L A A W H R L V L P I A Y E F N P E L V L V S A G F D A A R G D P L G G C Q V S P E G Y A H L T H L L M G L A S G R I I L I L E G G Y N L T S I S E S M A A C T R S L L G D P P P L L T L P R P P L S G A L A S I T E T I Q V H R R Y W R S L R V M K V E D R E G P S S S K L V T K K A P Q P A K P R L A E R M T T R E K K V L E A G M G K V T S A S F G E E S T P G Q T N S E T A V V A L T Q D Q P S E A A T G G A T L A Q T I S E A A I G G A M L G Q T T S E E A V G G A T P D Q T T S E E T V G G A I L D Q T T S E D A V G G A T L G Q T T S E E A V G G A T L A Q T T S E A A M E G A T L D Q T T S E E A P G G T E L I Q T P L A S S T D H Q T P P T S P V Q G T T P Q I S P S T L I G S L R T L E L G S E S Q G A S E S Q A P G E E N L L G E A A G G Q D M A D S M L M Q G S R G L T D Q A I F Y A V T P L P W C P H L V A V C P I P A A G L D V T Q P C G D C G T I Q E N W V C L S C Y Q V Y C G R Y I N G H M L Q H H G N S G H P L V L S Y I D L S A W C Y Y C Q A Y V H H Q A L L D V K N I A H Q N K F G E D M P H P H "
- Accession
- Q9UBN7
- Protein length
- Full Length
- Predicted molecular weight
- 161 kDa
- Amino acids
- 1 to 1215
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Protein was purified by affinity chromatography and gel filtration.
General info
- Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10220385). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10220385). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10220385). In addition to histones, deacetylates other proteins, such as CTTN, tubulin and SQSTM1 (PubMed:12024216, PubMed:20308065, PubMed:26246421, PubMed:30538141, PubMed:31857589). Plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin (PubMed:12024216, PubMed:20308065, PubMed:26246421). Required for cilia disassembly; via deacetylation of alpha-tubulin (PubMed:17604723, PubMed:26246421). Promotes deacetylation of CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy (PubMed:30538141). Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer (PubMed:24413532). Promotes odontoblast differentiation following IPO7-mediated nuclear import and subsequent repression of RUNX2 expression (By similarity). In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome (PubMed:17846173). Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy (PubMed:17846173).
- Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
- Post-translational modifications
Phosphorylated by AURKA; phosphorylation increases HDAC6-mediated deacetylation of alpha-tubulin and subsequent disassembly of cilia.
- Subcellular localisation
- Cytoskeleton, Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HDAC6 or histone deacetylase 6 is a protein that primarily functions as a cytoplasmic deacetylase. It is part of the class IIb HDAC family and is known for its distinctive molecular weight of approximately 121 kDa. HDAC6 is expressed in various tissues with higher levels observed in the brain kidney and liver. This protein is unique as it contains two catalytic domains unlike other HDACs which contributes to its specific deacetylation of non-histone substrates including tubulin and Hsp90 influencing cell motility and stress response.
- Biological function summary
HDAC6 plays a significant role in processes like protein degradation and cell signaling. It is an important component of the protein quality control system involving itself in the aggresome pathway where it facilitates the removal of misfolded proteins through interaction with dynein motor proteins. In addition to its presence in the cytoplasm HDAC6 influences cell migration and immune response regulation by de-phosphorylating cortactin and affecting actin filament dynamics. Its integral role in the aggresome-autophagy pathway positions it as important for cellular homeostasis maintenance.
- Pathways
HDAC6 participates prominently in both autophagy and stress response pathways. In the autophagic process HDAC6 operates alongside ubiquitinated proteins to manage protein quality control. Moreover HDAC6 engages in stress response pathways like the heat shock response interacting directly with Hsp90 to regulate client protein activation. These pathways highlight HDAC6’s relationships with key proteins such as Hsp70 and tau linking it to cellular stress and neurodegeneration responses.
- Associated diseases and disorders
HDAC6 exhibits connections to neurodegenerative diseases and cancer. Dysregulated HDAC6 activity associates with Alzheimer's disease where it affects tau protein accumulation and degradation. The protein is also implicated in various cancers such as breast and ovarian cancer due to its influence on cell migration and invasion. It interacts with p53 impacting apoptosis and tumor progression making HDAC6 a potential target for therapeutic interventions with HDAC6 inhibitors which aim to restore normal cellular functions disrupted by abnormal HDAC6 activity.
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2 product images
Functional Studies - Recombinant human HDAC6 protein (Active) (ab42632)
Specific Activity: ≥350 pmol/min/μg.
Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 3, and HDAC6 (3.1 – 200 ng). Incubation condition: 30 min at 37°C followed by developing for 15 min at room temperature.
SDS-PAGE - Recombinant human HDAC6 protein (Active) (ab42632)
4-20% SDS-PAGE - Recombinant human HDAC6 protein (Active) (ab42632).Lane 1: 2μg HDAC6.
Lane 2: MW marker.
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